%0 Journal Article
%T 产KPC酶超耐药阿斯肠杆菌EaH7的发现及其耐药伴生质粒的遗传特征
%A 崔嘉真
%A 黄建胜
%A 朱乃硕
%A △
%J 复旦大学（医学报）
%D 2015
%X </br>Objective To analyze the drug-resistant mechanism and genetic characteristics of a strain of imipenem-resistant Enterobacter asburiae. Methods The strain and its antibiotics minimum inhibitory concentration was identified by Vitek-2 Compact System, then it was determined by sequencing its 16s rRNA gene. Seventeen genes including β-lactamase resistance gene, quinolone resistance gene and aminoglycoside resistance gene were detected by PCR method and confirmed by sequencing. The plasmid of Enterobacter asburiae was transformed by CaCl 2-induced chemical method. Associated plasmid DNA library was constructed and sequenced. We annotated and predicted encoding function of the associated plasmid by Glimmer 3.02 and BLASTP. The role of the associated small plasmid on transferring to the drug-resistant plasmid was verified by conjugation. Results This strain belonged to Enterobacter asburiae.It was resistant to 15 kinds of β-lactam antibiotics, such as imipenem and aminoglycoside antibiotics, but it was sensitive to levofloxacin and ciprofloxacin. This strain contained kinds of plasmids.The drug-resistant plasmid pEa-1 carried resistance genes blaKPC-2, blaCTX-M-15 and blaTEM-1, while the associated plasmid pEa-2 carried 4 mobilization proteins genes called mob A, mob B, mob C and mob D.pEa-2 might promote the conjugation of drug-resistant plasmid pEa-1. Conclusions It is the first time to report that a strain of Enterobacter asburiae can produce KPC-2 enzyme in China, and plasmid pEa-2 carried by the strain might promote the conjugation of drug-resistant plasmid pEa-1.
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%K Enterobacter asburiae drug-resistant plasmid associated plasmid
%U http://jms.fudan.edu.cn/CN/abstract/abstract1188.shtml