%0 Journal Article
%T 表儿茶素没食子酸酯对人鼻咽癌C666-1细胞凋亡的影响<br>Effect of Epicatechin Gallate on the Apoptosis of Human Nasopharyngeal Carcinoma Cell Line C666-1
%A 蔡轶
%A 魏沁
%A 蔡跃鹏
%A 赵莉   &lt
%A br&gt
%A Cai Yi
%A Wei Qin
%A Cai Yuepeng
%A Zhao Li
%J 华南师范大学学报（自然科学版）
%D 2015
%R 10.6054/j.jscnun.2015.05.014
%X 该文观察ECG在人鼻咽癌细胞株C666-1增殖与凋亡中的作用，并初步探讨其机制.人鼻咽癌C666-1细胞给予不同浓度ECG（0~100 μmol/L）处理48 h后，采用CCK-8法检测细胞增殖水平，使用TUNEL法和流式细胞仪检测细胞凋亡率，利用Western blotting法检测Bax、Bcl-2、Caspase 3等凋亡相关蛋白的表达变化.结果表明，25 μmol/L以上浓度的ECG可抑制C666-1细胞增殖，诱导细胞凋亡；并且ECG可浓度依赖性增加Bax/Bcl-2蛋白比值和剪切的Caspase 3蛋白水平.以上结果表明ECG可抑制人鼻咽癌细胞株C666-1增殖，并诱导细胞凋亡.ECG的作用与其增加Bax/Bcl-2相对蛋白比值进而激活caspase-3有关.<br>： The effect of ECG on the proliferation and apoptosis of nasopharyngeal carcinoma cell line C666-1 were studied. C666-1 cells were treated with different concentrations (0~100 μmol/L) of ECG for 48 h, CCK-8 assay was used to evaluate the proliferation. TUNEL assay and Flow Cytometric Analysis were used to investigate the apoptosis of C666-1 cells. Western blotting was performed to detect the expression level of Bax, Bcl-2 and Caspase 3. ECG above 25 μmol/L could inhibit the proliferation, induce the apoptosis of C666-1 cells, and upregulate the levels of Bax/Bcl-2 and cleaved caspase 3. ECG could inhibit proliferation and induce apoptosis of nasopharyngeal carcinoma cell line C666-1 by regulating Bax-Bcl-2-Caspase 3 signaling pathway
%K 表儿茶素没食子酸酯
%K C666-1
%K 凋亡
%K &lt
%K br&gt
%K epicatechin gallate
%K C666-1
%K apoptosis
%U http://journal.scnu.edu.cn:8080/jwk_xbzrb/CN/abstract/abstract3628.shtml