%0 Journal Article
%T 机械通气对黏蛋白-5AC表达的影响及复方川贝精片的干预作用
%A 董琼
%A 邓安彦
%A 王波
%A 陈绍平
%A 蒲青
%A 陈艳
%J 华西医学
%P 871-873
%D 2011
%X 【】　目的　观察机械通气对黏蛋白（mucin，MUC）-5AC表达的影响及复方川贝精片的干预作用。　方法　新西兰兔25只，6个月龄，雄性；随机分为对照组、机械通气12h组及复方川贝精片低、中、高剂量组。收集支气管灌洗液，分别采用实时荧光定量聚合酶链式反应法和酶联免疫吸附试验检测支气管灌洗液中p38MAPKmRNA，MUC-5AC蛋白和mRNA的表达。　结果　机械通气能增强MUC-5AC的分泌（P<0.05）；加用复方川贝精片能降低机械通气后MUC-5AC蛋白和mRNA的表达（P<0.05）；复方川贝精片中、高剂量组与低剂量组比较，能降低机械通气后MUC-5AC蛋白和mRNA的表达（P<0.05）。　结论　机械通气能促进支气管黏膜上皮细胞分泌MUC-5AC，复方川贝精片能抑制机械通气所致MUC-5AC表达升高，其机制可能与其抑制p38MAPK表达有关。【Abstract】　Objective　Toobservetheeffectofmechanicalventilationbybreathingmachineontheexpressionofmucin(MUC-5AC)andtheinterferingeffectofcompoundtabletoffritillarybulb.　Methods　NewZealandRabbitswererandomlydividedintocontrolgroup,twelve-hourmechanicalventilationgroup,andlow,mediumandhigh-dosecompoundtabletoffritillarybulbgroup.Contentsofp38MAPKmRNA,MUC-5ACmRNAandproteininbronchialirrigatingsolutionweredetectedbyrealtimeRT-PCRandELISAmethods.　Results　MechanicalventilationcouldincreasetheexpressionofMUC-5ACinbronchialirrigatingsolution(P<0.05).CompoundtabletoffritillarybulbcoulddecreasetheexpressionofMUC-5ACmRNAandproteinaftermechanicalventilation(P<0.05).Comparedwithlow-dosecompoundtabletoffritillarybulbgroup,theexpressionofMUC-5ACmRNAandproteinwaslowerforthehighandmedium-dosegroups(P<0.05).　Conclusions　MechanicalventilationcanpromotetheexpressionofMUC-5ACinbronchialendothelialcells,whichcanbesuppressedbycompoundtabletoffritillarybulb.Thismaybeduetothesuppressioneffectofp38MAPKexpression.
%K 机械通气
%K 黏蛋白-5AC
%K 复方川贝精片
%K p38MAPK
%U http://www.hxyxqk.com.cn/oa/DArticle.aspx?type=view&id=201106020