%0 Journal Article
%T 晚期糖基化终产物对人结肠癌细胞SW-480增殖的影响及其机制研究
%A 周小智
%A 薛耀明
%A 关美萍
%A 朱波
%J 华西医学
%P 837-841
%D 2011
%X 【】　目的　观察晚期糖基化终产物（advancedglycosylationendprodrcts，AGE）对人结肠癌细胞株SW-480增殖的影响，并探讨其可能机制。　方法　不同浓度AGE干预SW-480细胞，噻唑蓝（MTT）法比较各组细胞活力，流式细胞术观察AGE对SW-480细胞周期的影响，蛋白质印迹法观察AGE对SW-480细胞CyclinD1表达的影响，端粒重复序列扩增法（telomericrepeatamplificationprotocol，TRAP）银染法观察AGE对SW-480细胞端粒酶活性的影响。MTT测细胞活力的检测设置空白对照组、100μg/mL小牛血清白蛋白（bovineserumalbumin，BSA）组及50、100、500μg/mLAGE组，其余检测只设置100μg/mLBSA组和100μg/mLAGE组。　结果　MTT结果示AGE促进SW-480细胞的增殖，且呈浓度依赖性。100μg/mLBSA组与100μg/mLAGE组72h后的细胞G0/G1期所占百分比分别为56.02%±0.58%、51.93%±1.01%，差异有统计学意义（P<0.05）。蛋白质印迹法示100μg/mLAGE组72h后CyclinD1的表达较100μg/mLBSA组增加，差异有统计学意义（P<0.05）。TRAP银染法检测示100μg/mLAGE干预SW-480细胞72h后可以增加端粒酶活性（P<0.05）。　结论　AGE可促进人结肠癌细胞SW-480生长，呈剂量依赖性。其作用机制可能与AGE上调CyclinD1的表达加速G1/S期转换及增加端粒酶活性有关。【Abstract】　Objective　Toobservetheeffectsofadvancedglycosylationendproducts(AGE)onproliferationofSW-480cellsandstudythepossiblemechanism.　Methods　VariousconcentrationsofAGEweredesignedtohaveimpactonSW-480cells.ProliferationofSW-480cellswasassessedbythiazolylbluetetrazoliumbromide(MTT)assay;TheimpactofAGEonthecellcycleofSW-480cellswasanalyzedbyflowcytometry(FCM);theinfluenceofAGEonexpressionofCyclinD1wascheckedbyWesternblotting;andtheimpactofAGEontelomeraseactivitywasexaminedbytelomericrepeatamplificationproctol(TRAP)sliverstaining.FortheMTTassay,blankcontrolgroup,100μg/mLbovineserumalbumin(BSA)group,50,100and500μg/mLAGEgroupsweredesigned,whileforotherexaminations,therewereonly100μg/mLBSAgroupand100μg/mLAGEgroup.　Results　MTTresultshowedthatAGEincreasedtheproliferationofSW-480cellsinadose-dependentmode.TheproportionofthecellsatG0/G1stageofthe100μg/mLBSAgroupandthe100μg/mLAGEexperimentalgroupwere(56.02±0.58)%and(51.93±1.01)%respectivelyafter72hours,withasignificantdifference(P<0.05);westernblottingshowedthattheexpressionofCyclinD1inthe100μg/mLAGEgroupwassignificantlyhigherthanthatinthe100μg/mLBSAgroupafter72hours;TRAPsilverstainingdemonstratedthattelomeraseactivityincreasedsignificantlyaftertreatedwith100μg/mLAGEfor72hours.　Conclusions　AGEcanpromotethegrowthofSW-480cellsinadose-dependentmode.Itsmechanismismainlybyup-regulatingtheexpressionofCyclinD1toshortenG0/G1andincreasingthetelomeraseactivitysignificantly.
%K 晚期糖基化终产物
%K SW-480细胞
%K 细胞周期
%K CyclinD1
%K 端粒酶活性
%U http://www.hxyxqk.com.cn/oa/DArticle.aspx?type=view&id=201106010