%0 Journal Article
%T EGFL7基因过度表达与喉癌分化、转移及预后的关系
%A 王晓侠
%A 姚小宝
%A 嵇宪生
%A 陈菁华
%A 李蕾
%A 朱宏亮
%J 华西医学
%P 710-713
%D 2011
%X 【】　目的　探讨喉癌组织中EGFL7基因mRNA表达与喉癌分化、转移及临床预后的关系。　方法　收集2008年5―11月共42例行喉癌手术患者的切除标本。用免疫组织化学法检测EGFL7蛋白在42例喉癌组织和配对癌旁组织中的表达情况，提取肿瘤及癌旁组织配对标本的总RNA，用逆转录（RT）-PCR方法测定EGFL7基因表达，蛋白质印迹法测定EGFL7蛋白的表达，并结合临床资料，对EGFL7基因差异表达与喉癌患者临床表现的相关性进行分析研究。　结果　对42例配对标本分别进行EGFL7mRNA荧光检测比较，30例标本的肿瘤组织中EGFL7基因mRNA表达明显高于癌旁正常喉组织。22例标本的肿瘤组织中EGFL7蛋白表达明显高于癌旁正常喉组织。EGFL7mRNA的表达与喉癌淋巴结转移和浸润深度密切相关（P0.05）。　结论　EGFL7基因在喉癌组织中表达状态与喉癌的生长和浸润转移关系密切，EGFL7基因可望作为喉癌病情发展及指导临床治疗的标记物之一。【Abstract】　Objective　ToinvestigatetheassociationofEGF-like-domain,multiple7(EGFL7)mRNAexpressionlevelwiththedifferentiation,metastasisandprognosisoflaryngocarcinoma.　Methods　Tissuespecimenswereobtainedfrom42patientsundergoingsurgeryforlaryngocarcinomabetweenMayandNovember,2008.ImmunohistochemistrywasusedtodetectthelevelofEGFL7inthe42tumortissuesandmatchedadjacentnormaltissues.ReversetranscriptionalPCR(RT-PCR)wasperformedforamplificationofEGFL7mRNAfromthe42tumortissuesandmatchedadjacentnormaltissues,andwesterblotwasadoptedtodetermineEGFL7proteinexpression.ThedifferentialEGFL7mRNAexpressionwasanalyzedforitsassociationwiththeclinicalmanifestationsofthepatients.　Results　EGFL7mRNAexpressionwasdetectedinallthelaryngocarcinomatissuesandnormaltissuesadjacenttothecarcinomausingfluorescencemethod.EGFL7mRNAexpressionwassignificantlyhigherinthetumortissuesthanintheadjacenttissuesin30cases,andEGFL7proteinexpressionwasalsosignificantlyhigherinthetumortissuesthanintheadjacentnormallaryngealtissuesin22cases.ExpressionofEGFL7mRNAwashighlycorrelatedwithlymphnodemetastasisandTclassification(P0.05).　Conclusions　EGFL7mRNAexpressioniscorrelatedcloselywiththedifferentiationandmetastasisoflaryngocarcinoma.EGFL7mayserveasamarkerforassessingtheprogressionoflaryngocarcinomaandprovideassistanceforclinicaltherapeuticdecisions.
%K 喉癌
%K EGFL7
%K 基因表达
%K 肿瘤转移
%K 预后
%U http://www.hxyxqk.com.cn/oa/DArticle.aspx?type=view&id=201105020