%0 Journal Article
%T NF-κB在重症急性胰腺炎小鼠肠黏膜屏障损伤中的作用
%A 张宇飞
%A 嵇武
%A 丁凯
%A 杨荣
%A 范铭兴
%A 刘兴东
%A 李秋荣
%J 华西医学
%P 1429-1432
%D 2010
%X 【】目的探讨NF-κB在重症急性胰腺炎小鼠肠黏膜屏障功能损伤中的调控机制。方法36只BALB/C小鼠随机分为对照组、模型组、NF-κB干预组，每组12只。18h后处死小鼠，比较各组的腹腔内大体改变、肠黏膜病理改变，肠道通透性的变化及血清细胞因子水平，肠上皮紧密连接蛋白occludin的表达。结果模型组小鼠腹腔内呈明显炎症反应，肠管水肿，肠黏膜水肿，肠道通透性显著增高，NF-κB特异性阻断剂能降低肠道损伤，改善肠黏膜水肿，上调肠上皮紧密连接蛋白occludin的表达，显著降低肠道通透性，降低细胞因子水平。结论NF-κB阻断剂能够通过选择性的抑制NF-κB活性，改善受损的肠屏障功能。这一作用通过上调肠上皮紧密连接蛋白occludin的水平而实现。？【Abstract】ObjectiveToinvestigatetherolesofNF-κBintheintestinalmucosalbarrierinjuryinmicewithsevereacutepancreatitis(SAP).MethodsThirty-sixBALB/Cmicewererandomlyassignedtonormalcontrolgroup,SAPmodelgroupandinterventiongroup.Eighteenhourslater,pathologicalintestinalvilluschanges,intestinalpermeability,serumcytokineswereevaluatedinallthreegroups.ResultsInSAPmodelgroup,intestinalmucosawasfoundtobeoedematousandintestinalpermeabilitywasmarkedlyincreased.NF-κBcouldameliorateintestinalinjuryandmucosaedema,andimproveintestinalpermeabilitybyupregulatingoccludingexpression.ConclusionNF-κBcouldprotectthefunctionofintestinalmucosalbarrierbyinhibitingNF-κBactivity,whichsuggeststhatNF-κBmayplayanintermediatingroleinSAP-inducedintestinalfailurethroughupregulatingoccludingexpression.
%K 重症急性胰腺炎
%K NF-κB
%K occludin
%K 肠黏膜屏障
%K 小鼠
%U http://www.hxyxqk.com.cn/oa/DArticle.aspx?type=view&id=201008014