%0 Journal Article
%T IdentificationofAnamorphYeastofTremellaaurantialbaandOptimizationofMediumCompositionforProductionofExopolysaccharides
%A 刘春卉
%A 杨秀伟
%A 俞建国
%A 马维新
%A 瞿伟菁
%A 包艳洁
%A 武文斌
%A 庄秀园
%J 过程工程学报
%D 2012
%X Ayeast-likefungusstrainB1isolatedfromwildfungusTremellaaurantialbawasidentifiedandinitiallycharacterized.TwophylogenetictreesweregeneratedbasedonthesequencesoflargesubunitribosomalRNAgeneD1/D2regionsandinternaltranscribedspacer(ITS)regionsofrelatedfungi,respectively.TheanalysisofD1/D2regionsandITSsequencesshowedthatfungusB1wasclusteredtogetherwithT.aurantialba,T.aurantiaandT.microsporeinthephylogenetictrees.BoththemorphologicalcharacteristicandphylogeneticanalysisestablishedthatfungusB1wasoneoftheanamorphstrainsofT.aurantialbaandbelongstoTremellagenus.Afermentationmediumforexopolysaccharides(EPS)productionbyT.aurantialbaB1.Plackett-Burmendesignwasusedtoevaluatetheeffectsofdifferentcomponentsintheculturemedium.GlucoseandyeastextracthavesignificantinfluenceontheEPSproduction.Theconcentrationsoftwofactorswereoptimizedsubsequentlyusingcentralcompositedesignandresponsesurfaceanalysis.Theresultsshowedthat49.2g/Lglucoseand10.4g/LyeastextractcouldleadtothemaximumproductionofEPS(4.99g/L).Theoptimizedmediumledtoa1.5-foldenhancementoftheproductionofEPSbyT.aurantialbaB1,ascomparedwiththatwithoutoptimization.
%K Tremellaaurantialba
%K anamorphyeast
%K largesubunitribosomalRNAgene
%K internaltranscribedspacer
%K exopolysaccharides
%K centralcompositedesign
%U http://www.jproeng.com/qikan/Cpaper/zhaiyao.asp?bsid=16708