%0 Journal Article
%T MetabolismKineticsofGlucoseinAnchorage-dependentCellCultures
%A SUNXiang-ming(孙祥明)
%A ZHANGYuan-xing(张元兴)
%J 过程工程学报
%D 2001
%X ThekineticmodelofglucosemetabolismwasestablishedandsuccessfullyappliedtobatchculturesofrCHOandrBHKcells.Itwasfoundthatalargeamountofglucosewasutilizedforcellmaintenance,andtheoverwhelmingmajorityofmaintenanceenergyfromglucosewasbyitsanaerobicmetabolisminbothrBHKandrCHOcellcultures.Theoverallmaintenancecoefficientsfromaerobicmetabolismwere1.9?10-13mmol/(cell？h)forrCHOcellsand7?10-13mmol/(cell？h)forrBHKcells.Inaddition,allGO/TandEO/TgraduallyincreasedwiththesametrendasthecellgrowthinthecultureofbothrCHOandrBHKcells.Theoverallmoleculeyieldcoefficientsoflactatetoglucosewere1.61forrCHOcellsand1.38forrBHKcells.Theyieldcoefficientsofcelltoglucosewere4.5?108cells/mmolforrCHOcellsand1.9?108cells/mmolforrBHKcells,respectively.
%K animalcellculture
%K metabolism
%K kinetics
%K glucose
%K maintenanceenergy
%U http://www.jproeng.com/qikan/Cpaper/zhaiyao.asp?bsid=997