%0 Journal Article
%T Separation,PurificationandCharacterizationofThreeEndo-polygalacturonasesfromaNewlyIsolatedPenicillumoxalicum
%A 张春辉
%A 李祖明
%A 彭霞薇
%A 贾月
%A 张洪勋
%A 白志辉
%J 过程工程学报
%D 2009
%X Threeendo-polygalacturonases(endoPGs)fromanewlyisolatedPenicillumoxalicum(CGMCC0907)capableofutilizingwastebiomassasgrowthsubstratewereseparatedandpurifiedtohomogeneitybyultra-filtration,affinityadsorptionchromatography,CM-cellulosecolumnchromatography,andSephadexG-100gelfiltrationchromatographywiththeoverallyieldof64.5%fromthecrudeenzyme.ThespecificactivitiesandrecoveryratesofendoPG-1,endoPG-2andendoPG-3were1120U/mgand21.6%,1350U/mgand25.9%,and1560U/mgand17.0%,respectively.ThethreepurifiedendoPGshadaclosemolecularweightto41kDaasestimatedbySDS-PAGE.TheoptimumtemperatureandpHforthefunctionofthemwere65℃and5.0,55℃and5.0,50℃and5.5,respectively.TheirpIandKmvalueswere5.9and0.78mg/mL,6.0and1.2mg/mL,and6.1and2.0mg/mL,respectively.
%K separation
%K characterization
%K Penicillumoxalicum
%K endo-polygalacturonase
%U http://www.jproeng.com/qikan/Cpaper/zhaiyao.asp?bsid=15124