%0 Journal Article
%T A sensitive, rapid and validated liquid chromatography ? tandem mass spectrometry (LC-MS-MS) method for determination of Mimosine in Mimosa pudica Linn
%A Parikshit A. Champanerkar
%A Vikas V. Vaidya
%A Sunita Shailajan
%A Sasikumar N. Menon
%J Natural Science
%P 713-717
%@ 2150-4105
%D 2010
%I Scientific Research Publishing
%R 10.4236/ns.2010.27088
%X A rapid, sensitive and accurate liquid chroma-tographic tandem mass spectrometric method is described for the determination of Mimosine in Mimosa pudica Linn. whole plant powder. Mi-mosine was extracted from the plant using 1.0% HCl in water. The chromatographic separation was achieved using a Thermo Hypurity C18 (50 x 4.6 mm) 5.0 ¦Ì column interfaced with a triple quadrapole mass spectrometer. The mobile phase consisted of a mixture of Methanol: 10 mM ammonium formate buffer whose pH was ad-justed to 3.00 ¡À 0.05 with formic acid (80:20, v/v) and was delivered at a flow rate of 1.0 mL min-1. Electrospray ionization (ESI) source operated in the negative ion mode was used for the quantitation. Detection was performed using an Applied Biosystems Sciex API 3200 Mass spec-trometer. The method was found to be simple, precise, accurate, fast, specific and sensitive and can be used for routine quality control analysis of Mimosine in Mimosa pudica Linn.
%K LC-MS-MS
%K Mimosine
%K Mimosa pudica Linn
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=2302