%0 Journal Article
%T Use of Cryopreserved Osteogenic Matrix Cell Sheets for Bone Reconstruction
%A Tomohiko Kura
%A Manabu Akahane
%A Takamasa Shimizu
%A Yoshinobu Uchihara
%A Yasuaki Tohma
%A Yusuke Morita
%A Munehisa Koizumi
%A Kenji Kawate
%A Yasuhito Tanaka
%J Stem Cell Discovery
%P 13-23
%@ 2161-6787
%D 2016
%I Scientific Research Publishing
%R 10.4236/scd.2016.61002
%X Skeletal diseases, such as nonunion and osteonecrosis, are now treatable with tissue engineering techniques. Single cell sheets called osteogenic matrix cell sheets (OMCSs) grown from cultured bone marrow-derived mesenchymal stem cells show high osteogenic potential; however, long preparation times currently limit their clinical application. Here, we report a cryopreservation OMCS transplantation method that shortens OMCS preparation time. Cryopreserved rat OMCSs were prepared using slow- and rapid-freezing methods, thawed, and subsequently injected scaffold-free into subcutaneous sites. Rapid- and slow-frozen OMCSs were also transplanted directly to the femur bone at sites of injury. Slow-freezing resulted in higher cell viability than rapid freezing, yet all two cryopreservation methods yielded OMCSs that survived and formed bone tissue. In the rapid- and slow-freezing groups, cortical gaps were repaired and bone continuity was observed within 6 weeks of OMCS transplantation. Moreover, while no significant difference was found in osteocalcin expression between the three experimental groups, the biomechanical strength of femurs treated with slow-frozen OMCSs was significantly greater than those of non-transplant at 6 weeks post-injury. Collectively, these data suggest that slow-frozen OMCSs have superior osteogenic potential and are better suited to produce a mineralized matrix and repair sites of bone injury.
%K Bone Marrow Stromal Cell
%K Cryopreservation
%K Cell Sheet
%K Injectable Bone
%K Bone Reconstruction
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=62609