%0 Journal Article
%T 伏马菌素B1胶体金免疫层析试纸条的研制
%A 樊海新
%A 李寒松
%A 李复辉
%A 马良友
%A 朱平
%A 吴金节
%A 王希春
%J 南京农业大学学报
%D 2015
%R 10.7685/j.issn.1000-2030.2015.03.020
%X [目的] 建立用于快速检测伏马菌素B1(FB1)的胶体金免疫层析试纸条。[方法] 首先制备了FB1单克隆抗体和FB1-OVA偶联抗原,然后将FB1单克隆抗体与胶体金制成金标抗体包被在金标垫上,将FB1-OVA抗原和羊抗鼠IgG包被在硝酸纤维素膜(NC膜)表面分别作为检测线和质控线。采用间接竞争法检测待检样品中的FB1与NC膜上的FB1-OVA抗原竞争结合金标抗体中的FB1单克隆抗体情况,并以检测线和质控线显示检测结果。[结果] 本试验优化了金标抗体、检测抗原及羊抗鼠IgG的包被量,检测FB1的灵敏度可达到2 ng？mL-1,最佳判定质量浓度为40 ng？mL-1。[结论] 本试验制备的检测试纸条具有较高的特异性和稳定性,操作简单,检测时间仅需10 min,且与高效液相色谱法检测结果一致,可作为检测玉米中伏马菌素B1残留的有效手段。</br>[Objectives] A rapid golden immunochromatographic strip for the detection of fumonisin B1(FB1)was developed. [Methods] Specific monoclonal antibody against FB1 and FB1-OVA conjugate antigen was prepared. FB1 monoclonal antibody, labeled with colloidal gold, was used as probes on the immunochromatographic strip. FB1-OVA and goat anti-mouse IgG were coated onto nitrocellulose membrane as test lines and control lines, respectively. FB1 in sample will competitively combined the FB1 monoclonal antibody with the FB1-OVA in nitrocellulose filter membrance(NC) and the result will be observed by the color of detection line and quality control line directly. [Results] In this study, the concentrations of FB1 monoclonal antibody labeled with colloidal gold, detecting antigen and goat anti-mouse IgG were optimized. During the detection of corn, the lowest detectable limit reaches 2 ng？mL-1, and the best determination concentration reaches 40 ng？mL-1. The test can be completed only within 10 min. [Conclusions] The detection of samples showed that the developed method had a positive correlation with the HPLC method. It can be an effective method to detect FB1 in corns
%K 伏马菌素B1
%K 单克隆抗体
%K 胶体金免疫层析试纸条&lt
%K /br&gt
%K fumonisin B1
%K monoclonal antibody
%K colloidal gold immunochromatographic strip
%U http://nauxb.njau.edu.cn/oa/darticle.aspx?type=view&id=201503020