%0 Journal Article
%T 三丁酸甘油酯对宫内发育迟缓哺乳仔猪肝脏发育和免疫功能的影响
%A 何进田
%A 董丽
%A 张莉莉
%A 寇涛
%A 白凯文
%A 黄雪新
%A 刘春雪
%A 王恬
%J 南京农业大学学报
%D 2015
%R 10.7685/j.issn.1000-2030.2015.05.020
%X [目的]研究三丁酸甘油酯对宫内发育迟缓(IUGR)哺乳仔猪肝脏发育及免疫功能的影响.[方法]选用24头新生(杜洛克×长白×大白)三元杂交哺乳仔猪,分为3个处理组,8头正常新生仔猪为正常(NBW)组,16头IUGR仔猪随机均分为IUGR组和IT组,NBW组和IUGR组饲喂基础人工乳,IT组饲喂基础人工乳+0.10%三丁酸甘油酯.21日龄屠宰取样.[结果]IUGR组肝脏质量显著低于NBW组(P <0.05);IT组肝脏质量显著高于IUGR组(P <0.05),且IT组肝脏指数显著高于IUGR组和NBW组(P <0.05).IUGR组肝脏胰岛素样生长因子1(IGF-1)含量显著低于NBW组和IT组(P <0.05),其含量在IT组与NBW组间差异不显著(P >0.05).IUGR组肝脏肿瘤坏死因子α(TNF-α)、白细胞介素4(IL-4)含量均显著高于NBW组和IT组(P <0.05),而IT组与NBW组间差异不显著(P >0.05).IUGR组肝脏IGF-1 mRNA的表达显著低于NBW组和IT组(P <0.05).IUGR组肝脏饥饿激素mRNA的表达显著高于NBW和IT组(P <0.05),但在IT组与NBW组间没有显著差异(P >0.05).IUGR组肝脏白细胞介素1β(IL-1β)、IL-4 mRNA的表达水平均极显著高于NBW组(P <0.01),但IT组IL-1β mRNA的表达水平极显著低于NBW组(P <0.01),而IT组IL-4 mRNA的表达水平极显著高于NBW组(P <0.01).[结论]IUGR会限制哺乳仔猪肝脏发育,引起肝脏发生炎症反应.在哺乳期饲喂三丁酸甘油酯可以促进肝脏发育,提高肝脏指数和有效缓解肝脏炎症反应,增强IUGR哺乳仔猪肝脏的免疫功能.</br>[Objectives]This experiment was conducted to study the effects of tributyrin on the development and immune function of the liver in the intrauterine growth restricted(IUGR) suckling piglets. [Methods]24 new born piglets(Duroc×Landrace×Yorkshine)were chosen and assigned to three treatment groups. 8 normal birth weight piglets were allocated to normal birth weight(NBW)group,and 16 IUGR piglets were randomly assigned into IUGR group and IT(IUGR+tributyrin)group. All piglets were weaned at 7 day of age,and were fed with the artificial milk diets from day 7 to 21. Piglets in the NBW group and the IUGR group were fed with basic milk diets,while piglets in the IT group were fed with basic milk diets supplemented with 0.10% tributyrin. 6 piglets in each group were selected and sacrificed on day 21. [Results]The results showed that:1)The liver weight of the piglets in the IUGR group was smaller than that of the NBW group(P <0.05);the liver weight of the piglets in the IT group was higher than that of IUGR group(P <0.05)and the liver index of IT group was significantly higher than that of IUGR group and NBW group(P <0.05). 2)The content of insulin growth factor-1(IGF-1)in the liver of the piglets in the IUGR group was significantly lower than that in NBW group and IT group(P <0.05),but the content of IGF-1 in IT group was higher than that in NBW group(P >0.05). The contents of tumor necrosis factor-α(TNF-α),interleukin-4(IL-4)in the liver of the piglets in the IUGR group were significantly higher than those of NBW group and IT group(P <0.05),but there were no significant differences between IT and NBW groups(P >0.05). 3)The expression of IGF-1 mRNA in the liver of the piglets in the IUGR group was significantly lower than that in the NBW group and IT group(P
%K 三丁酸甘油酯
%K IUGR哺乳仔猪
%K 肝脏
%K 发育
%K 免疫功能&lt
%K /br&gt
%K tributyrin
%K IUGR suckling piglets
%K liver
%K development
%K immune function
%U http://nauxb.njau.edu.cn/oa/darticle.aspx?type=view&id=201505020