%0 Journal Article
%T Cytotoxicityandgenome-widemicroarrayanalysisofintestinalsmoothmusclecellsinresponsetohexavalentchromiuminduction
%A Li-Fang JIN
%A Yuan-Yuan WANG
%A Zi-Dong ZHANG
%A Yi-Meng YUAN
%A Yi-Rui HU
%A Yang-Feng WEI
%A Jian NI
%J 动物学研究
%D 2013
%R 10.11813/j.issn.0254-5853.2013.E3.E93
%X Chronicingestionofhighconcentrationsofhexavalentchromium[Cr(VI)]indrinkingwaterinducesintestinaltumorsinmice;however,informationonitstoxicityonintestinalsmoothmusclecellsislimited.ThepresentstudyaimedtoassesstheinvitroandinvivotoxicologicaleffectsofCr(VI)onintestinalsmoothmusclecells.Humanintestinalsmoothmusclecells(HISMcells)wereculturedwithdifferentconcentrationsofCr(VI)toevaluateeffectsoncellproliferationability,oxidativestresslevels,andantioxidantsystem.Furthermore,tissuesectionsinCr(VI)exposedrabbitswereanalyzedtoevaluatetoxicityonintestinalmusclecellsinvivo.Genechipswereutilizedtoassessdifferentialgeneexpressionprofilesatthegenome-widelevelin1μmol/LCr(VI)treatedcells.IntestinaltissuebiopsyresultsshowedthatCr(VI)increasedtheincidencesofdiffuseepithelialhyperplasiainintestinaljejunumbutcausednoobviousdamagetothestructureofthemuscularis.Cellproliferationanalysisrevealedthathighconcentrations(≥64μmol/L)butnotlowconcentrationsofCr(VI)(≤16μmol/L)significantlyinhibitedthegrowthofHISMcells.Foroxidativestresslevels,theexpressionofreactiveoxygenspecies(ROS)andnitricoxide(NO)waselevatedathighconcentrations(≥64μmol/L)butnotatlowconcentrationsofCr(VI)(≤16μmol/L).Inaddition,dose-dependentincreasesintheactivityofoxidizedglutathione(GSSH)/total-glutathione(T-GSH)werealsoobserved.Genechipscreened491differentiallyexpressedgenesincludinggenesassociatedwithcellapoptosis,oxidations,andcytoskeletons.SomeofthesedifferentiallyexpressedgenesmaybeuniquetosmoothmusclecellsinresponsetoCr(VI)induction.
%K Intestinalsmoothmusclecells
%K Hexavalentchromium
%K Cytotoxicity
%K Genechip
%U http://www.zoores.ac.cn/CN/abstract/abstract3350.shtml