%0 Journal Article
%T Characterizationof12polymorphicmicrosatellitemarkersintheChinesetreeshrew(Tupaiabelangerichinensis)
%A Xiao-Hong LIU
%A Yong-Gang YAO
%J 动物学研究
%D 2013
%R 10.3724/SP.J.1141.2013.E02E62
%X TheChinesetreeshrew(Tupaiabelangerichinensis)isasmallexperimentalanimalwithacloseaffinitytoprimates.Thisspecieshaslongbeenproposedtobeanalternativeexperimentalanimaltoprimatesinbiomedicalresearch.Despitedecadesofstudy,thereisnopurebreedforthisanimal,andtheoverallgeneticdiversityofwildtreeshrewsremainslargelyunknown.Inordertoobtainasetofgeneticmarkersforevaluatingthegeneticdiversityoftreeshrewwildpopulationsandtracingthelineagesininbreedingpopulations,wedeveloped12polymorphicmicrosatellitemarkersfromthegenomicDNAofthetreeshrew.Ananalysisofawildpopulationof117individualscollectedfromthesuburbofKunming,China,showedthattheselociexhibitedahighlyexpectedheterozygosity(0.616).These12microsatellitesweresufficientforindividualidentificationandparentageanalysis.Themicrosatellitemarkersdevelopedinthisstudywillbeofuseinevaluatinggeneticdiversityandlineagetracingforthetreeshrew.
%K Chinesetreeshrew
%K Microsatellite
%K Heterozygosity
%K Individualidentification
%K Parentagetesting
%U http://www.zoores.ac.cn/CN/abstract/abstract3320.shtml