%0 Journal Article
%T mRNAexpressionandDNAmethylationinthreekeygenesinvolvedincastedifferentiationinfemalehoneybees(Apismellifera)
%A Xin-Liang SHAO
%A Shao-Yu HE
%A Xin-Ying ZHUANG
%A Ying FAN
%A Ya-Hui LI
%A Yong-Gang YAO
%J 动物学研究
%P 92-98
%D 2014
%R 10.11813/j.issn.0254-5853.2014.2.092
%X Inhoneybee(Apismellifera)colonies,queensandworkersarealternativeformsoftheadultfemalehoneybeethatdevelopfromgeneticallyidenticalzygotesbutthatdependondifferentialnourishment.Queensandworkersdisplaydistinctmorphologies,anatomiesandbehavior,betterknownascastedifferentiation.Despitesomebasicinsights,theexactmechanismresponsibleforthisphenomenon,especiallyatthemolecularlevel,remainsunclearalthoughsomeprogresshasbeenachieved.Inthisstudy,weexaminedmRNAlevelsoftheTOR(targetofrapamycin)andDnmt3(DNAmethyltransferase3)genes,closelyrelatedtocastedifferentiationinhoneybees.WealsoinvestigatedmRNAexpressionoftheS6K(similartoRPS6-p70-proteinkinase)genelinkedcloselytoorganismalgrowthanddevelopmentinqueenandworkerlarvae(1-dayand3-dayold).Last,weinvestigatedthemethylationstatusofthesethreegenesincorrespondingcastes.WefoundnodifferenceinmRNAexpressionforthethreegenesbetween1stinstarqueenandworkerlarvae;however,3rdinstarqueenlarvaehadahigherlevelofTORmRNAthanworkerlarvae.Methylationlevelsofallthreegeneswerelowerinqueenlarvaethanworkerlarvaebutthedifferenceswerenotstatisticallysignificant.Thesefindingsprovidebasicdataforbroadeningourunderstandingofcastedifferentiationinfemalehoneybees.
%K Castedifferentiation
%K DNAmethylation
%K DNAmethyltransferase3
%K Honeybee
%K Targetofrapamycin
%U http://www.zoores.ac.cn/CN/abstract/abstract3443.shtml