%0 Journal Article
%T SequencingandphylogeneticanalysisofthePyrgilaudaruficollis(Aves,Passeridae)completemitochondrialgenome
%A Yong-Gui MA
%A Yuan HUANG
%A Fu-Min LEI
%J 动物学研究
%P 81-91
%D 2014
%R 10.11813/j.issn.0254-5853.2014.2.081
%X Inthisstudy,bothlongPCRandconservedprimerswalkingsequencingmethodswereusedtodeterminethecompletesequenceoftheofPyrgilaudaruficollismitochondrialgenome(KC836121).TheresultsshowedthatthecompletemitochondrialgenomeofP.ruficollisis16909bpinlengthwith55.0%A+Tcontent,harboringthetypical37genes.ThemitogenomehadthesamegeneorderwiththatofPodoceshendersoni.AllproteincodinggenesstartedwithATGcodon,exceptND3withGTG.Forthestopcodonusage,mostgenesterminatewithcodonsTAAorTAG,butND5terminatedwithAGA,whileND1andCOIgeneswithAGG,andboththegenesCOⅢandND4haveanincompleteterminationcodon(T).Thesecondarystructuresof22tRNAgeneswerealsopredicted,showingthatalltRNAscanformtypicalclover-leafsecondarystructures,exceptforthetRNASer(AGN)whichlosestheDHUarm,whiletRNAPheharboranextranucleotideinsertedintheTψCarm.Thepredictedsecondarystructuresof12SrRNAand16SrRNAexhibit47helicesin4domainsand60helicesin6domainsrespectively.ThecontrolregionofP.ruficolliswiththelengthof1305bpwaslocatedbetweentRNAGluandtRNAPhe,andtypicaldomainsofwhichcouldbefoundasotherbirdgroups.Usingthedatafrom13mitochondrialprotein-codinggenes,resultsofafinalphylogeneticanalysisstronglysupportsthetraditionalviewthatP.ruficollisiscloselyrelatedwithPasseridaeandFringillidae.
%K Pyrgilaudaruficollis
%K Completemitochondrialgenome
%K Phylogeny
%U http://www.zoores.ac.cn/CN/abstract/abstract3442.shtml