%0 Journal Article
%T PurificationandcharacterizationofcholecystokininfromtheskinofsalamanderTylototritonverrucosus
%A Wen-Bin JIANG
%A Ma HAKIM
%A Lei LUO
%A Bo-Wen LI
%A Shi-Long YANG
%A Yu-Zhu SONG
%A Ren LAI
%A Qiu-Min LU
%J 动物学研究
%P 174-177
%D 2015
%X Asagroupofintestinalhormonesandneurotransmitters,cholecystokinins(CCKs)regulateandaffectpancreaticenzymesecretion,gastrointestinalmotility,painhypersensitivity,digestionandsatiety,andgenerallycontainaDYMGWMDFGsequenceattheC-terminus.ManyCCKshavebeenreportedinmammals.However,onlyafewhavebeenreportedinamphibians,suchasHylanigrovittata,Xenopuslaevis,andRanacatesbeiana,withnonereportedinurodeleamphibianslikenewtsandsalamanders.Here,aCCKcalledCCK-TVwasidentifiedandcharacterizedfromtheskinofthesalamanderTylototritonverrucosus.ThisCCKcontainedanaminoacidsequenceofDYMGWMDF-NH2asseeninotherCCKs.AcDNAencodingtheCCKprecursorcontaining129aminoacidresidueswasclonedfromthecDNAlibraryofT.verrucosusskin.TheCCK-TVhadthepotentialtoinducethecontractionofsmoothmusclestripsisolatedfromporcinegallbladder,elicitingcontractionataconcentrationof5.0x10-11mol/Landinducingmaximalcontractionataconcentrationof2.0x10-6mol/L.TheEC50was13.6nmol/L.Tothebestofourknowledge,thisisthefirstreporttoidentifythepresenceofaCCKinanurodeleamphibian.
%K Cholecystokinin
%K Salamander
%K Skin
%K Amphibian
%U http://www.zoores.ac.cn/CN/abstract/abstract3644.shtml