%0 Journal Article
%T EstablishmentofHIV-1modelcelllineGHOST(3)withstableDRiP78andNHERF1knockdown
%A Lin ZHANG
%A Xu-He HUANG
%A Ping-Ping ZHOU
%A Guo-Long YU
%A Jin YAN
%A Bing QIN
%A Xin-Ge YAN
%A Li-Mei DIAO
%A Peng LIN
%A Yi-Qun KUANG
%J 动物学研究
%P 161-166
%D 2015
%X ChemokinereceptorsCXCR4andCCR5areindispensableco-receptorsforHIV-1entryintohostcells.Inourpreviousstudy,weidentifiedthatdopaminereceptor-interactingprotein78(DRiP78)andNa+-H+exchangerregulatoryfactor1(NHERF1)aretheCXCR4andCCR5homo-orhetero-dimer-interactingproteins.DRiP78andNHERF1areabletoinfluencetheco-receptorinternalizationandintracellulartrafficking.Over-expressionofNHERF1affectstheligandsorHIV-1gp120-inducedCCR5internalizationandHIV-1production.ItisreasonabletospeculatethatDRiP78andNHERF1,aswellasthesignalingpathwaysinvolvedinviralreplication,wouldprobablyaffectHIV-1replicationthroughregulatingtheco-receptors.Inthispresentstudy,wedesignedtwoshorthairpinRNAs(shRNAs)targetingtheDRiP78andNHERF1,respectively,andconstructedthepLenti6/BLOCK-iT-DESTlentiviralplasmidsexpressingDRiP78orNHERF1shRNA.Thepackagedlentiviruseswereusedtotransducethewidely-appliedHIV-1modelcelllineGHOST(3).Then,cellswithstableknockdownwereestablishedthroughselectingtransducedcellswithBlasticidin.Thisstudy,forthefirsttime,reportedtheestablishmentoftheGHOST(3)withDRiP78andNHERF1knockdown,whichisthefirststablecelllinewithHIV-1co-receptor-interactingmoleculardefects.
%K HIV-1
%K DRiP78
%K NHERF1
%K shRNA
%K GHOST(3)cells
%U http://www.zoores.ac.cn/CN/abstract/abstract3642.shtml