%0 Journal Article
%T SelectiverecruitmentofhostfactorsbyHSV-1replicationcenters
%A Feng-Chao LANG
%A Xin LI
%A Olga VLADMIROVA
%A Zhuo-Ran LI
%A Gui-Jun CHEN
%A Yu XIAO
%A Li-Hong LI
%A Dan-Feng LU
%A Hong-Bo HAN
%A Ju-Min ZHOU
%J 动物学研究
%P 142-151
%D 2015
%X Herpessimplexvirustype1(HSV-1)entersproductiveinfectionafterinfectingepithelialcells,whereitcontrolsthehostnucleustomakeviralproteins,startsviralDNAsynthesisandassemblesinfectiousvirions.Inthisprocess,replicatingviralgenomesareorganizedintoreplicationcenterstofacilitateviralgrowth.HSV-1isknowntousehostfactors,includinghostchromatinandhosttranscriptionregulators,totranscribeitsgenes;however,theinvadingvirusalsoencountershostdefenseandstressresponsestoinhibitviralgrowth.Recently,wefoundthatHSV-1replicationcentersrecruithostfactorCTCFbutexcludeγH2A.X.Thus,HSV-1replicationcentersmayselectivelyrecruitcellularfactorsneededforviralgrowth,whileexcludinghostfactorsthataredeleteriousforviraltranscriptionorreplication.HerewereportthattheviralreplicationcentersselectivelyexcludedmodifiedhistoneH3,includingheterochromatinmarkH3K9me3,H3S10PandactivechromatinmarkH3K4me3,butnotunmodifiedH3.WefoundadynamicassociationbetweentheviralreplicationcentersandhostRNApolymeraseII.ThecentersalsorecruitedcomponentsoftheDNAdamageresponsepathway,including53BP1,BRCA1andhostantiviralproteinSP100.Importantly,wefoundthatATMkinasewasneededfortherecruitmentofCTCFtotheviralcenters.TheseresultssuggestthattheHSV-1replicationcenterstookadvantageofhostsignalingpathwaystoactivelyrecruitorexcludehostfactorstobenefitviralgrowth.
%K HSV-1
%K CTCF
%K &gamma
%K H2AX
%K Viralreplicationcente
%K RNAPolII
%U http://www.zoores.ac.cn/CN/abstract/abstract3640.shtml