%0 Journal Article
%T EffectsofalcoholonH3K9acetylationinmousepre-implantationembryos
%A Fang DING
%A Li CHEN
%A Yong LIU
%A Feng-Rui WU
%A Biao DING
%A Wen-Yong LI
%A Rong WANG
%J 动物学研究
%P 54-58
%D 2015
%R 10.13918/j.issn.2095-8137.2015.1.54
%X Itiswellknownthatexcessivelong-termalcoholconsumptionisharmful,especiallyinpregnantwomen.Inthepresentstudy,theKunmingwhitemousewasusedasananimalmodelandindirectimmunofluorescencewasperformedtoanalyzethetoxiceffectsofalcoholonearlypre-implantationembryos.H3K9acetylationimmunofluorescencecouldnotbedetectedinMIIoocytes.H3K9acetylationlevelsinthetreatmentgroupwerehigherthaninthecontrolgroupduringthemorulastage,andcontrarytoresultsduringtheblastocyststage.Otherstagesshowednoobviousdifferencesforinvivoembryos.Forinvitroembryos,almostnodifferencewasfoundbetweenthetwoexperimentalgroupsacrossallstages,andbothgroupsshowedincreasingH3K9acetylationlevels(exceptatthe2-cellstage).ThisstudyshowsthatH3K9acetylationlevelsinearlypre-implantationembryosarenotablyimpactedbyexcessivealcoholingestionbyfemales.Thesedataarethefirststepinunderstandingtheepigeneticmechanismofalcoholtoxicityinearlypre-implantationmouseembryos.
%K Alcoholintake
%K Pre-implantationembryos
%K H3K9acetylation
%K Mice
%K Embryodevelopment
%U http://www.zoores.ac.cn/CN/abstract/abstract3563.shtml