%0 Journal Article
%T ThecomingofRNA-basedpestcontrols
%A Xu Linghua
%A Zeng Baosheng
%A Noland Jeffery
%A Huang Yongping
%A Zhou Xuguo
%J 植物保护学报
%P 673-690
%D 2015
%X RNAinterference(RNAi)isagenesilencingtoolthattargetsmessengerRNA(mRNA)transcriptsinasequencespecificmanneranddownregulatesgeneexpressionbyinteractingwithsmallinterferingRNAs(siRNAs).mRNAscanbesilencedeitherthroughendogenousdegradationvianucleaseactivity(e.g.,RNAipathway),orbyinhibitingtranslation(e.g.,miRNA).Overthepastdecade,RNAihasbeenusedbroadlyinentomologicalresearchtodecipherthegenefunctionsininsects.WiththesuccessofRNAiinfunctionalgenomicsresearch,muchattentionhasshiftedtothepotentialapplicationsofRNAiinagriculture,especiallyforthecontrolofinsectpests.InadditiontoRNAi-basedgenesilencing,genomeediting,anexcitingnewbiotechnology,offersyetanotheroptionforpestcontrols.Themodificationofplantgenomestoknock-ingenesthatareheritabletoincreasethetoleranceofplantstoinsectinfestation,orknock-outgenesinpestinsectsthroughgeneticallymodified(GM)-insectreleasesareontheforefrontofthegenetic-basedpestmanagements.Inthisreview,wesummarizethecurrentknowledgeregardingRNAi-basedgenesilencingandCRISPR/Cas9-basedgenomeediting.TechnicalchallengesandregulatoryconcernsforthisnewwaveofRNA-basedpestcontrolsarediscussedingreatdetail.WealsosharetheperspectiveofmodifyingcurrentenvironmentalriskassessmentframeworkstobetterfittheRNA-basedpestmanagementstrategies.Giventhecurrentdiscussions/attentionsoverthesafetyofGeneticallyModifiedOrganisms(GMO),we,respectively,comparedprosandconsofRNAi-basedgenesilencingandCRISPR/Gas9-basedgenomeediting,andlaterweidentifiedtheregulatoryissuesthatshouldbeaddressedbeforetheseemergingbiotechnologiescanmovefromthebenchtoptothetabletop.
%K RNAi
%K genomeediting
%K CRISPR/Cas9
%K genesilencing
%K pestcontrol
%K technicalchallenges
%K regulatoryhurdles
%K emergingbiotechnology
%K ecologicalriskassessment
%U http://www.zwbhxb.com.cn/ch/reader/view_abstract.aspx?file_no=20150501&flag=1