%0 Journal Article
%T 黄连解毒汤有效成分对缺氧/复氧时脑微血管内皮细胞的保护作用
%A 袁拯忠
%A 朱陵群
%A 庞鹤
%A 单泽松
%A 王硕仁
%A 高永红
%A 牛福玲
%J 中国中药杂志
%D 2007
%X 目的:研究栀子苷、黄芩苷和小檗碱对大鼠脑微血管内皮细胞的保护作用。方法:建立离体大鼠脑微血管内皮细胞缺氧/复氧损伤模型,用1.024,0.512,0.256,0.128,0.064,0.032,0.016,0.008μmol.mL-1栀子苷,0.224,0.112,0.056,0.028,0.014,0.007,0.003μmol.mL-1黄芩苷和0.192,0.096,0.048,0.024,0.012,0.006,0.003μmol.mL-1小檗碱分别作用于正常组和模型组细胞。细胞活性用MTT比色法检测。结果:0.128,0.064,0.032μmol.mL-1栀子苷,0.028,0.014,0.007μmol.mL-1黄芩苷和0.024,0.012,0.006μmol.mL-1小檗碱能够比较理想的保护缺氧4h复氧12h损伤的大鼠脑微血管内皮细胞。结论:适当浓度的栀子苷、黄芩苷和小檗碱等黄连解毒汤主要成分可以保护脑微血管内皮细胞。
%K 黄连解毒汤
%K 脑微血管内皮细胞
%K 缺氧/复氧
%K 栀子苷
%K 黄芩苷
%K 小檗碱
%U http://www.cjcmm.com.cn/cjcmm/ch/reader/view_abstract.aspx?file_no=512&flag=1