%0 Journal Article
%T 胡萝卜籽抗氧化肽的分离纯化及活性研究<br>Purification and characterization of carrot seed-derived sntioxidant peptide
%A 户
%A 佩
%A 胡海峰
%A 陈涛涛
%A 陈小燕
%A 张
%A 杨
%A 洪
%A 晶
%J 福州大学学报(自然科学版)
%D 2015
%R 10.7631/issn.1000-2243.2015.06.0846
%X 采用凝胶过滤色谱Sephadex G-50和反相高效液相色谱对胡萝卜籽中的抗氧化肽进行分离纯化.以DPPH自由基（2，2-二苯基-1-苦肼基）清除率为指标，测定多肽的抗氧化活性.采用电喷雾电离质谱法测定该多肽的相对分子质量. 研究结果表明： 经过分离纯化，得到一个具有较强抗氧化活性的多肽，在质量浓度为1 mg·mL-1时，其DPPH自由基清除活力达到（92.02±0.47）%，相对分子质量为339.3 u，由3个氨基酸残基组成.<br>In the present study，the antioxidant peptides from carrot seeds were purified by size exclusion chromatography Sephadex G-50 and RP-HPLC (reverse-phase high performance liquid chromatography). The antioxidant activity of each fraction was evaluated by DPPH (2，2-diphenyl-1-picrylhydrazyl) radical-scavenging effect. The molecular mass of the antioxidant peptide was identified by electrospray ionization mass spectrometry (ESI-MS). Finally，an antioxidant peptide with a molecular mass of 339.3 u was obtained. It exhibited strong DPPH radical-scavenging capability，and the scavenging rates reached to (92.02±0.47)% at the concentration of 1 mg·mL-1. The results indicated that carrot seed-derived peptides showed strong antioxidant activity
%K 胡萝卜籽 多肽 纯化 抗氧化 高效液相色谱&lt
%K br&gt
%K carrot seeds peptide purification antioxidant HPLC
%U http://xbzrb.fzu.edu.cn/ch/reader/view_abstract.aspx?file_no=20150621&flag=1