%0 Journal Article
%T 悬浮固化液相微萃取-高效液相色谱法分析生长素<br>Analysis of auxins by solidification of floating organic drop combined with high-performance liquid chromatography
%A 卢巧梅
%A 张文敏
%A 黄川辉
%A 卢明华
%A 励建荣
%A 张
%A 兰
%J 福州大学学报(自然科学版)
%D 2015
%X 建立基于悬浮固化液相微萃取预处理的高效液相色谱-荧光检测法（SFODME-HPLC-FLD）用于两种植物生长素（吲哚丙酸和吲哚丁酸）的分析. 实验考察各因素对萃取过程的影响，确定最佳条件：50 μL十二醇（纯）为萃取剂，20.0 mL样品溶液（pH 3.0）、0.25 g·mL-1 NaCl、26 ℃、1 800 r·min-1条件下萃取10 min. 结果表明，SFODME技术重现性好、灵敏度高，相对标准偏差小于8.66%，检测限为0.01 ng·mL-1，线性范围为0.02~60 ng·mL-1，加标回收率大于84.3%. 方法快速、稳定、环境友好，适用于实际样品的检测.<br>Determination of two anxins (indole propionic acid and indole butyric acid) by solidification of floated organic drop microextraction with high-performance liquid chromatography-fluorescence detection (SFODME-HPLC-FLD) was established. The experimental conditions affecting the extraction process were optimized. The optimal conditions of SFODME were as follows：50 μL dodecanol as extraction solvent，20.0 mL sample solution with pH 3.0 and 0.25 g·mL-1 NaCl. The above solution was extracted for 10 min at temperature of 26 ℃ and centrifugation speed of 1 800 r·min-1. Results showed that this SFODME approach had high sensitivity and good reproducibility，with relative standard deviations lower than 8.66%. Its linear range was 0.02~60 ng·mL-1，with the limit of detection of 0.01 ng·mL-1 and the recoveries larger than 84.3%. This method was rapid，stable and environmentally benign，and it can be applied to real sample analysis
%K 悬浮固化液相微萃取 高效液相色谱 液相微萃取 生长素&lt
%K br&gt
%K solidified floating organic drop microextraction high-performance liquid chromatography liquid phase microextraction auxin
%U http://xbzrb.fzu.edu.cn/ch/reader/view_abstract.aspx?file_no=20150422&flag=1