%0 Journal Article
%T p16, Cyclin D1, and HIF-1¦Á Predict Outcomes of Patients with Oropharyngeal Squamous Cell Carcinoma Treated with Definitive Intensity-Modulated Radiation Therapy
%A Asal S. Rahimi
%A David D. Wilson
%A Drew K. Saylor
%A Edward B. Stelow
%A Christopher Y. Thomas
%A James F. Reibel
%A Paul A. Levine
%A David C. Shonka
%A Mark J. Jameson
%A Paul W. Read
%J International Journal of Otolaryngology
%D 2012
%I Hindawi Publishing Corporation
%R 10.1155/2012/685951
%X We evaluated a panel of 8 immunohistochemical biomarkers as predictors of clinical response to definitive intensity-modulated radiotherapy in patients with oropharyngeal squamous cell carcinoma (OPSCC). 106 patients with OPSCC were treated to a total dose of 66–70 Gy and retrospectively analyzed for locoregional control (LRC), disease-free survival (DFS), and overall survival (OS). All tumors had p16 immunohistochemical staining, and 101 tumors also had epidermal growth factor receptor (EGFR) staining. 53% of the patients had sufficient archived pathologic specimens for incorporation into a tissue microarray for immunohistochemical analysis for cyclophilin B, cyclin D1, p21, hypoxia-inducible factor-1α (HIF-1α), carbonic anhydrase, and major vault protein. Median followup was 27.2 months. 66% of the tumors were p16 positive, and 34% were p16 negative. On univariate analysis, the following correlations were statistically significant: p16 positive staining with higher LRC (=0.005) and longer DFS (<0.001); cyclin D1 positive staining with lower LRC (=0.033) and shorter DFS (=0.002); HIF-1α positive staining with shorter DFS (=0.039). On multivariate analysis, p16 was the only significant independent predictor of DFS (=0.023). After immunohistochemical examination of a panel of 8 biomarkers, our study could only verify p16 as an independent prognostic factor in OPSCC.
%U http://www.hindawi.com/journals/ijol/2012/685951/