%0 Journal Article
%T Protein sliding and hopping kinetics on DNA
%A Michael C. DeSantis
%A Je-Luen Li
%A Y. M. Wang
%J Quantitative Biology
%D 2010
%I arXiv
%R 10.1016/j.bpj.2010.12.1268
%X Using Monte-Carlo simulations, we deconvolved the sliding and hopping kinetics of GFP-LacI proteins on elongated DNA from their experimentally observed seconds-long diffusion trajectories. Our simulations suggest the following results: (1) in each diffusion trajectory, a protein makes on average hundreds of alternating slides and hops with a mean sliding time of several tens of ms; (2) sliding dominates the root mean square displacement of fast diffusion trajectories, whereas hopping dominates slow ones; (3) flow and variations in salt concentration have limited effects on hopping kinetics, while in vivo DNA configuration is not expected to influence sliding kinetics; furthermore, (4) the rate of occurrence for hops longer than 200 nm agrees with experimental data for EcoRV proteins.
%U http://arxiv.org/abs/1010.3247v2