%0 Journal Article %T Pixel multiplexing for high-speed multi-resolution fluorescence imaging %A Gil Bub %A Matthias Tecza %A Michiel Helmes %A Peter Lee %A Peter Kohl %J Quantitative Biology %D 2009 %I arXiv %X We introduce a imaging modality that works by transiently masking image-subregions during a single exposure of a CCD frame. By offsetting subregion exposure time, temporal information is embedded within each stored frame, allowing simultaneous acquisition of a full high spatial resolution image and a high-speed image sequence without increasing bandwidth. The technique is demonstrated by imaging calcium transients in heart cells at 250 Hz with a 10 Hz megapixel camera. %U http://arxiv.org/abs/0910.0789v1