%0 Journal Article
%T Purification of an Exopolygalacturonase from Penicillium viridicatum RFC3 Produced in Submerged Fermentation
%A Eleni Gomes
%A Rodrigo Sim es Ribeiro Leite
%A Roberto da Silva
%A D¨ºnis Silva
%J International Journal of Microbiology
%D 2009
%I Hindawi Publishing Corporation
%R 10.1155/2009/631942
%X An exo-PG obtained from Penicillium viridicatum in submerged fermentation was purified to homogeneity. The apparent molecular weight of the enzyme was 92 kDa, optimum pH and temperature for activity were pH 5 and 50–55¡ãC. The exo-PG showed a profile of an exo-polygalacturonase, releasing galacturonic acid by hydrolysis of pectin with a high degree of esterification (D.E.). Ions Ca2
%U http://www.hindawi.com/journals/ijmb/2009/631942/