%0 Journal Article
%T Activation of Peroxisome Proliferator-Activated Receptor-Gamma by Glitazones Reduces the Expression and Release of Monocyte Chemoattractant Protein-1 in Human Mesothelial Cells
%A Matthias Sauter
%A Kathrin Kastenm¨ıller
%A Franziska Belling
%A Markus W rnle
%A Roland Ladurner
%A Thomas Mussack
%A Thomas Sitter
%J Mediators of Inflammation
%D 2012
%I Hindawi Publishing Corporation
%R 10.1155/2012/217696
%X Human peritoneal mesothelial cells (MC) play an important role in inflammatory processes of the peritoneal cavity by producing various cytokines and chemokines, such as monocyte chemoattractant protein-1 (MCP-1). The present study was designed to assess the effect of the peroxisome proliferator-activated receptor-gamma- (PPAR-) activator rosiglitazone on the mesothelial MCP-1 expression and release. Primary cultures of MC were obtained from omental tissue. MCP-1 antigen concentrations were measured in the cell supernatant by ELISA and MCP-1 mRNA levels by real-time polymerase chain reaction. The presence of PPAR on MC was assayed in a Western Blot analysis. MC constitutively express PPAR. Activation of this receptor via rosiglitazone (0,1–10 μmol/L) resulted in significantly reduced amounts of mesothelial MCP-1 release as well as MCP-1 mRNA. The use of the PPAR inhibitor GW-9662 could completely prevent the rosiglitazone effects. Rosiglitazone was also effective in reducing TNF-induced enhanced secretion of MCP-1. Our findings indicate that glitazones are effective in reducing constitutive and TNF-stimulated mesothelial MCP-1 mRNA expression and release.
%U http://www.hindawi.com/journals/mi/2012/217696/