%0 Journal Article
%T Clinical Significance and Expression of PAF and TNF-alpha in Seminal Plasma of Leukocytospermic Patients
%A Chaodong Liu
%A Hongjian Liu
%A Xianzhong Wang
%A Sun Xinbo
%J Mediators of Inflammation
%D 2012
%I Hindawi Publishing Corporation
%R 10.1155/2012/639735
%X Objective. Discuss the changes and roles of PAF in the reproductive tract infection by observing the expression of platelet activating factor (PAF) and tumor necrosis factor ¦Á (TNF-¦Á) in seminal plasma of patients with leukocytospermia. Methods. The seminal plasma was obtained from 22 cases of leukocytospermia and 15 cases of normal males; the peroxidase dyeing method was adopted for seminal plasma white blood count; the ELISA was adopted to test PAF and TNF-¦Á concentration in seminal plasma. Result. PAF concentration ( £¿ng/mL) of leukocytospermia group was significantly lower than the normal group ( £¿ng/mL, ) while TNF-¦Á ( £¿ng/mL) was significantly higher than that of normal group ( £¿ng/mL). There was negative correlation between PAF and TNF-¦Á , ( , ); the same situation existed in PAF and WBC ( , ); but TNF-¦Á was positively correlated to WBC ( , ). Conclusion. (1) Low expression of PAF and high expression of TNF-¦Á in leukocytospermia affect the sperm motility, which is one of the reasons that leads to infertility. (2) Lower expression of PAF has its particularity during the reproductive tract infection. 1. Introduction Leukocytospermia is a kind of common disease of male reproductive tract infection, which is usually caused by bacterial infection. An increase of white blood cells in seminal plasma can lead to the decrease of sperm quality and the increasing number of inflammatory cytokines such as interleukin IL-6 is the main factor involved in the process [1]. According to current studies, PAF is a kind of cytokine which has many kinds of biological activities, such as inflammation factors and sperm cell activation factors. How does the PAF change and what role does it play the role during the reproductive tract infection? The purpose of our study was to observe the expression of PAF and TNF-¦Á in seminal plasma of patients with leukocytospermia. 2. Material and Method 2.1. Research Population Male patients age from 23 to 40 years old, who came to the Urology Surgical Department of our hospital from April to September 2011, were enrolled. According to WHO directives, 22 cases with leukocytospermia and 15 normal males were chosen. All the patients did not have any urinary, reproductive, blood, or endocrine diseases. The patients who have ever suffered from mumps, cryptorchidism and sexual dysfunction and ever accepted radiotherapy or chemotherapy were excluded. All the indexes were in the normal range according to seminal plasma regular analysis. The study was admitted by the local ethical committee, and written informed consent was obtained from all
%U http://www.hindawi.com/journals/mi/2012/639735/