%0 Journal Article %T Spike Sorting of Muscle Spindle Afferent Nerve Activity Recorded with Thin-Film Intrafascicular Electrodes %A Milan Djilas %A Christine Azevedo-Coste %A David Guiraud %A Ken Yoshida %J Computational Intelligence and Neuroscience %D 2010 %I Hindawi Publishing Corporation %R 10.1155/2010/836346 %X Afferent muscle spindle activity in response to passive muscle stretch was recorded in vivo using thin-film longitudinal intrafascicular electrodes. A neural spike detection and classification scheme was developed for the purpose of separating activity of primary and secondary muscle spindle afferents. The algorithm is based on the multiscale continuous wavelet transform using complex wavelets. The detection scheme outperforms the commonly used threshold detection, especially with recordings having low signal-to-noise ratio. Results of classification of units indicate that the developed classifier is able to isolate activity having linear relationship with muscle length, which is a step towards online model-based estimation of muscle length that can be used in a closed-loop functional electrical stimulation system with natural sensory feedback. 1. Introduction Functional electrical stimulation (FES) is one solution for restoring movement in paralyzed limbs. In order to correct for disturbances and unavoidable musculoskeletal modeling errors, closed-loop FES is desirable. With the advent of advanced implanted prosthetic interfaces, natural sensors are being explored as an alternative source for feedback information. By directly interfacing the peripheral nerves, it is possible to record signals from natural sensors distributed throughout the body [1¨C4]. Muscle spindles are one type of natural sensor. Their main function is to signal changes in the length of the muscle within which they reside [5]. Changes in the muscle length are associated with changes in the angles of the joints that the muscles cross. Therefore, their activity can be used to provide feedback information about muscle state in a closed-loop FES system [6], as these sensors remain intact and active below the level of lesion in spinal cord injured patients [7]. In a number of studies muscle spindle afferent activity was recorded using cuff electrodes to provide natural sensory feedback [1, 4, 8¨C10]. Despite their chronic stability and noise immunity, the use of cuff electrodes is ultimately limited by their low selectivity, which requires them to be distributed throughout the body and in close proximity to the sensory end organs targeted for use in feedback [11]. An alternative to the cuff is the longitudinal intrafascicular electrode (LIFE). It is designed to be implanted longitudinally within the peripheral nerve where it can record activity from a relatively small population of nerve fibers [12¨C16]. In a previous study a simple corrector controller was implemented to follow a desired %U http://www.hindawi.com/journals/cin/2010/836346/