%0 Journal Article
%T MolecularCloningofaCytosolicG6PDHGenefromPopulussuaveolensandItsExpressiontoImprovetheColdResistanceofTobaccoPlants
%A LINYuan-zhen
%A GUOHai
%A ZHANGZhi-yi
%A LINShan-zhi
%J 冰川冻土
%P 1178-1185
%D 2009
%I 科学出版社
%X Glucose-6-phosphatedehydrogenase(G6PDH,EC1.1.1.49)isthefirstandmainregulatedenzymeofoxidativepentosephosphatepathway(OPPP),catalyzingtheconversionofglucose-6-phosphateto6-phospho-gluconolactoneandplayingimportantrolesinthegrowthanddevelopmentofplants.WepreciouslyreportedthattheenhancementoffreezingresistanceofPopulussuaveolenscuttingswasclearrelatedtothedistinctincreaseincytosolicG6PDHactivity.Here,a1697bpcDNAfragment(PsG6PDH)wasamplifiedbyRT-PCRfromcold-inducedtotalRNAofthefreezing-tolerantP.suaveolens.ThesequenceanalysisshowedthatPsG6PDHcodingregionhad1530bpandencoded510predictedaminoacidresidues.GenomicSouthernanalysisrevealedthattheisoformisencodedbyafewcopiesofthegeneinthepoplargenome.TheclonedgenePsG6PDHwasclonedintobinaryvectorpBI121andusedtotransformtobacco.PCRandSouthernblottingresultsverifiedintegrationofthisgeneintothegenomeoftobacco.Moreover,coldtreatmentexperimentsandmembranedefenseenzymeactivityanalysisconfirmedthatoverexpressionofthePsG6PDHgenecouldenhancethetolerancetocoldorfrigidstressesintransgenicplants.
%K Populus
%K suaveolens
%K glucose-6-phosphate
%K dehydrogenase
%K cold
%K resistance
%K gene
%K engineering
%U http://bcdt.westgis.ac.cn/CN/abstract/abstract221.shtml