%0 Journal Article
%T TGF-¦Â Suppression of HBV RNA through AID-Dependent Recruitment of an RNA Exosome Complex
%A Guoxin Liang&nbsp
%A Guangyan Liu&nbsp
%A Kouichi Kitamura&nbsp
%A Zhe Wang&nbsp
%A Sajeda Chowdhury&nbsp
%A Ahasan Md Monjurul&nbsp
%A Kousho Wakae&nbsp
%A Miki Koura&nbsp
%A Miyuki Shimadu&nbsp
%A Kazuo Kinoshita
%J PLOS Pathogens
%D 2015
%I Public Library of Science (PLoS)
%R 10.1371/journal.ppat.1004780
%X Transforming growth factor (TGF)-¦Â inhibits hepatitis B virus (HBV) replication although the intracellular effectors involved are not determined. Here, we report that reduction of HBV transcripts by TGF-¦Â is dependent on AID expression, which significantly decreases both HBV transcripts and viral DNA, resulting in inhibition of viral replication. Immunoprecipitation reveals that AID physically associates with viral P protein that binds to specific virus RNA sequence called epsilon. AID also binds to an RNA degradation complex (RNA exosome proteins), indicating that AID, RNA exosome, and P protein form an RNP complex. Suppression of HBV transcripts by TGF-¦Â was abrogated by depletion of either AID or RNA exosome components, suggesting that AID and the RNA exosome involve in TGF-¦Â mediated suppression of HBV RNA. Moreover, AID-mediated HBV reduction does not occur when P protein is disrupted or when viral transcription is inhibited. These results suggest that induced expression of AID by TGF-¦Â causes recruitment of the RNA exosome to viral RNP complex and the RNA exosome degrades HBV RNA in a transcription-coupled manner.
%U http://www.plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1004780