%0 Journal Article
%T Validation of an Easy Acetonitrile Fractionation for the Simplification of Protein Samples Prior to Proteomics Analyses
%A Yannis Karamanos
%A Barbara Deracinois
%A Gwenael Pottiez
%A Sophie Duban-Deweer
%J Open Access Library Journal
%V 2
%N 10
%P 1-6
%@ 2333-9721
%D 2015
%I Open Access Library
%R 10.4236/oalib.1101928
%X In most proteomics analyses and in particular for the ˇ°off-gelˇ±
approaches, based essentially on chromatography, the complexity of the proteome
should be reduced; otherwise identifications can be hindered, especially if the
mass spectrometry analysis is not conducted using state-of-the-art
instrumentation. Even if the method used is a bottom-up proteomics, it appears
mandatory to pre-fractionate the proteins in order to reduce the complexity. We
report here the development and validation of a pre-fractionation based on the
differential solubilisation of proteins using increasing concentrations of
acetonitrile (ACN). This ˇ°ACN fractionationˇ± was applied to the study of the
Triton X-100 soluble sub-proteome of brain capillary endothelial cells (BCEC)
with re-induced blood-brain barrier (BBB) functions.
%K Proteomics
%K Acetonitrile
%K Fractionation
%U http://www.oalib.com/paper/3150568