%0 Journal Article
%T Inhibitory Effect of ¦Á-Pinene on SGC-7901 Cell Proliferation and the Mechanism of ATM Kinase Signaling Pathway
%A Fuhong Zhu
%A Fengxiang Wei
%A Cui Zhang
%J Chinese Medicine
%P 27-33
%@ 2151-1926
%D 2015
%I Scientific Research Publishing
%R 10.4236/cm.2015.61004
%X <p style=\"text-align:justify;\">
	<span style=\"font-size:10.0pt;font-family:;\" \"=\"\"><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">Objective: To research the inhibitory effect on SGC-7901 cells of </span><i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">¦Á</span></i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">-pinene, and
the related mechanism of </span><i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">¦Á</span></i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">-pinene.
Methods: Used the MTT method to detect inhibition rate and western blotting to
detect the influence on expression of ATM, Phos-S1981ATM, H2AX, </span><i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">¦Ã</span></i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">H2AX, CHK2 and p-CHK2, p53 and phos-p53
cell cycle related protein in SGC-7901 cells. Results: The research found </span><i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">¦Á</span></i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">-pinene could inhibit the proliferation
of SGC-7901 cells observably </span><i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">in vitro</span></i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">,
and the inhibition rate assumes the dependence on concentration; and western
blotting results showed that, </span><i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">¦Á</span></i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">-pinene
could activate phospho-ATM, increase the amount of </span><i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">¦Ã</span></i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">H2AX (</span><i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">p </span></i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">&lt; 0.05, </span><i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">p </span></i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">&lt; 0.05); increase the expression of
p-CHK2, p53 and phos-p53 (</span><i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">p </span></i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">&lt; 0.05, </span><i><span style=\"font-family:Verdana;line-height:1.5;font-size:10px;\">p </span></i><span
%K &lt
%K i&gt
%K ¦Á&lt
%K /i&gt
%K -Pinene
%K SGC-7901 Cells
%K ATM Kinase
%K Proliferation
%K Cell Cycle
%K DNA Damage
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=54541