%0 Journal Article
%T Simple and Rapid Liquid Chromatography Method for Determination of Rifabutin in Plasma
%A AK Hemanth Kumar
%A V Sudha
%A Geetha Ramachandran
%J SAARC Journal of Tuberculosis, Lung Diseases and HIV/AIDS
%P 26-29
%D 2012
%R 10.3126/saarctb.v9i2.7975
%X A high performance liquid chromatographic method for determination of rifabutin in human plasma was  developed. The method involved deproteinisation of the sample with acetonitrile and analysis of the  supernatant using a reversed-phase C 18 column (250mm) and UV detection at a wavelength of 265nm.  The assay was specific for rifabutin and linear from 0.025 to 10.0μg/ml. The relative standard deviation  of intra- and inter-day assays was lower than 10%. The method was able to remove interfering materials  in plasma, yielding an average recovery of rifabutin from plasma of 101%. Due to its simplicity, the assay  can be used for pharmacokinetic studies of rifabutin. SAARC Journal of Tuberculosis, Lung Diseases & HIV/AIDS; 2012; IX(2) 26-29 DOI: http://dx.doi.org/10.3126/saarctb.v9i2.7975
%K tuberculosis
%K rifabutin
%K liquid chromatography
%U http://www.nepjol.info/index.php/SAARCTB/article/view/7975