%0 Journal Article
%T Imaging of Fluoride Ion in Living Cells and Tissues with a Two-Photon Ratiometric Fluorescence Probe
%A Xinyue Zhu
%A Jianxi Wang
%A Jianjian Zhang
%A Zhenjie Chen
%A Haixia Zhang
%A Xiaoyu Zhang
%J Sensors
%P 1611-1622
%D 2015
%I MDPI AG
%R 10.3390/s150101611
%X A reaction-based two-photon (TP) ratiometric fluorescence probe Z2 has been developed and successfully applied to detect and image fluoride ion in living cells and tissues. The Z2 probe was designed designed to utilize an ICT mechanism between n-butylnaphthalimide as a fluorophore and tert-butyldiphenylsilane (TBDPS) as a response group. Upon addition of fluoride ion, the Si-O bond in the Z2 would be cleaved, and then a stronger electron-donating group was released. The fluorescent changes at 450 and 540 nm, respectively, made it possible to achieve ratiometric fluorescence detection. The results indicated that the Z2 could ratiometrically detect and image fluoride ion in living cells and tissues in a depth of 250 ¦Ìm by two-photon microscopy (TPM).
%K two-photon excited
%K ratiometric fluorescence
%K imaging
%K fluoride
%U http://www.mdpi.com/1424-8220/15/1/1611