%0 Journal Article
%T Accelerated Detection of Viral Particles by Combining AC Electric Field Effects and Micro-Raman Spectroscopy
%A Matthew Robert Tomkins
%A David Shiqi Liao
%A Aristides Docoslis
%J Sensors
%P 1047-1059
%D 2015
%I MDPI AG
%R 10.3390/s150101047
%X A detection method that combines electric field-assisted virus capture on antibody-decorated surfaces with the ˇ°fingerprintingˇ± capabilities of micro-Raman spectroscopy is demonstrated for the case of M13 virus in water. The proof-of-principle surface mapping of model bioparticles (protein coated polystyrene spheres) captured by an AC electric field between planar microelectrodes is presented with a methodology for analyzing the resulting spectra by comparing relative peak intensities. The same principle is applied to dielectrophoretically captured M13 phage particles whose presence is indirectly confirmed with micro-Raman spectroscopy using NeutrAvidin-Cy3 as a labeling molecule. It is concluded that the combination of electrokinetically driven virus sampling and micro-Raman based signal transduction provides a promising approach for time-efficient and in situ detection of viruses.
%K micro-Raman spectroscopy
%K AC electrokinetics
%K dielectrophoresis
%K microelectrodes
%K optical biosensors
%K M13 phage
%U http://www.mdpi.com/1424-8220/15/1/1047