%0 Journal Article
%T Preparative Separation of Sulfur-Containing Diketopiperazines from Marine Fungus Cladosporium sp. Using High-Speed Counter-Current Chromatography in Stepwise Elution Mode
%A Binbin Gu
%A Yanying Zhang
%A Lijian Ding
%A Shan He
%A Bin Wu
%A Junde Dong
%A Peng Zhu
%A Juanjuan Chen
%A Jinrong Zhang
%A Xiaojun Yan
%J Marine Drugs
%P 354-365
%D 2015
%I MDPI AG
%R 10.3390/md13010354
%X High-speed counter-current chromatography (HSCCC) was successively applied to the separation of three sulfur-containing diketopiperazines (DKPs) (including two new compounds cladosporin A ( 1) and cladosporin B ( 3), and a known compound haematocin ( 2)) from a marine fungus Cladosporium sp. The two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water at (1:1:1:1, v/v) and (2:1:2:1, v/v), in stepwise elution mode, was used for HSCCC. The preparative HSCCC separation was performed on 300 mg of crude sample yielding 26.7 mg of compound 3 at a purity of over 95%, 53.6 mg of a mixture of compounds 1 and 2, which was further separated by preparative-HPLC yielding 14.3 mg of compound 1 and 25.4 mg of compound 2 each at a purity of over 95%. Their structures were established by spectroscopic methods. The sulfur-containing DKPs suppressed the proliferation of hepatocellular carcinoma cell line HepG2. The present work represents the first application of HSCCC in the efficient preparation of marine fungal natural products.
%K Cladosporium
%K high-speed counter-current chromatography
%K marine fungus
%K sulfur-containing diketopiperazines
%U http://www.mdpi.com/1660-3397/13/1/354