%0 Journal Article
%T Caffeic Acid-PLGA Conjugate to Design Protein Drug Delivery Systems Stable to Irradiation
%A Francesca Selmin
%A Francesco Puoci
%A Ortensia I. Parisi
%A Silvia Franz¨¦
%A Umberto M. Musazzi
%A Francesco Cilurzo
%J Journal of Functional Biomaterials
%P 1-13
%D 2015
%I MDPI AG
%R 10.3390/jfb6010001
%X This work reports the feasibility of caffeic acid grafted PLGA (g-CA-PLGA) to design biodegradable sterile microspheres for the delivery of proteins. Ovalbumin (OVA) was selected as model compound because of its sensitiveness of ¦Ã-radiation. The adopted grafting procedure allowed us to obtain a material with good free radical scavenging properties, without a significant modification of Mw and Tg of the starting PLGA ( Mw PLGA = 26.3 ¡À 1.3 kDa vs. Mw g-CA-PLGA = 22.8 ¡À 0.7 kDa; Tg PLGA = 47.7 ¡À 0.8 ¡ãC vs. Tg g-CA-PLGA = 47.4 ¡À 0.2 ¡ãC). By using a W 1/O/W 2 technique, g-CA-PLGA improved the encapsulation efficiency ( EE), suggesting that the presence of caffeic residues improved the compatibility between components ( EE PLGA = 35.0% ¡À 0.7% vs. EE g-CA-PLGA = 95.6% ¡À 2.7%). Microspheres particle size distribution ranged from 15 to 50 ¦Ìm. The zeta-potential values of placebo and loaded microspheres were £¿25 mV and £¿15 mV, respectively. The irradiation of g-CA-PLGA at the dose of 25 kGy caused a less than 1% variation of Mw and the degradation patterns of the non-irradiated and irradiated microspheres were superimposable. The OVA content in g-CA-PLGA microspheres decreased to a lower extent with respect to PLGA microspheres. These results suggest that g-CA-PLGA is a promising biodegradable material to microencapsulate biological drugs.
%K anti-oxidant
%K microencapsulation
%K drug release
%K caffeic acid
%K grafting
%K ¦Ã-irradiation
%K microspheres
%K ovalbumin
%K poly(lactide-co-glycolide)
%K sterilization
%U http://www.mdpi.com/2079-4983/6/1/1