%0 Journal Article
%T AgCu Bimetallic Nanoparticles under Effect of Low Intensity Ultrasound: The Cell Viability Study In Vitro
%A Vladan Bernard
%A Ond£¿ej Zoba£¿
%A Ji£¿¨ª Sopou£¿ek
%A Vojt¨§ch Mornstein
%J Journal of Cancer Research
%D 2014
%R 10.1155/2014/971769
%X The effects of metallic nanoparticles as cytotoxicity or antibacterial activity are widely known. It is also obvious that ultrasound is one of the most widely used therapeutic modalities in medicine. The effect of application of therapeutical ultrasonic field in the presence of metallic nanoparticles AgCu <100£¿nm modified by phenanthroline or polyvinyl alcohol was examined on human ovarian carcinoma cells A2780. Metallic nanoparticles were characterized by electron microscopy and by measuring of zeta potential. The cell viability was tested by MTT test. The experimental results indicate a significant decrease of cell viability, which was affected by a combined action of ultrasound field and AgCu nanoparticles. The maximum decrease of cells viability was observed for nanoparticles modified by phenanthroline. The effect of metallic nanoparticles on human cell in presence of ultrasound exposure was found¡ªa potential health risk or medical advantage of targeted therapy in the future. 1. Introduction Ultrasound is widely used in medicine and represents a large percentage of all imaging methods. Ultrasound is also used in therapy. For this reason it is necessary to study the effects of ultrasound on biological objects, although it is generally considered a modality with a very low risk of damage to the patient. The main mechanisms of biological action of ultrasonic energy on biological systems include cavitation, increase in temperature, and mechanical stress [1]. This paper shows that it is possible to observe changes due to ultrasound at the cellular level, for example, as changes in the porosity of cell membranes [2]. It is clear that the state of these structures in specific cases affects the uptake of active substances into the intracellular space. The possibility of targeted delivery of substances into cells using ultrasonic field has been established, both in drugs and in other macromolecular substances [3, 4]. Targeted synergetic effect of the ultrasound therapy and drugs is used in the sonodynamic therapy [5]. The still unsolved question is the effect of ultrasound on penetration of particles of nanometer scale into the intracellular space. This question is generally associated with particular risks of toxicity of nanoparticles, but it can be seen as a solution to some cancer treatment in the form of targeted therapy. Authors indicating the possibility of targeted nanoparticle delivery stimulated by ultrasound are not numerous and moreover they deal mainly with focused ultrasound of high intensity [6, 7]. The mechanism of ultrasound action in the
%U http://www.hindawi.com/journals/jcr/2014/971769/