%0 Journal Article
%T Effects of Exogenous Growth Hormone on Growth Hormone-Insulin-Like Growth Factor Axis of Human Gastric Cancer Cell
%A Daoming Liang
%A Yi Zhang
%A Jiayong Chen
%A Hua Wang
%A Tao Huang
%A Xin Xue
%J Chinese Medicine
%P 259-269
%@ 2151-1926
%D 2014
%I Scientific Research Publishing
%R 10.4236/cm.2014.54031
%X Aim: To study effects of recombinant human growth hormone (rhGH) on growth hormone-insulin-like growth factor axis (GH-IGFs) of human gastric cancer cell in vivo in order to reveal part mechanism of growth effects of rhGH on gastric cancer. Methods: Nude mice were randomly divided into control group, cisplatin (DDP) group, rhGH group and DDP + rhGH group after human gastric cancer xenograft model of node mice was successfully founded and drugs were used for 6 days. We investigated volume of tumor, inhibitory rate of tumor and cell cycle by slide gauge and flow cytometry. In addition, We also respectively investigated insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-3 (IGFBP-3) of blood serum of nude mice, IGF-ImRNA, insulin-like growth factor-I receptor (IGF-IR) mRNA and IGFBP-3 mRNA of xenograft of nude mice by enzyme linked immunosorbent assay (ELISA) and semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) on the first day of completing use of drugs later. Results: Tumor grew obviously slowly and tumor inhibitory rate obviously rose in DDP group and DDP + rhGH group compared with control group and rhGH group (<i>p</i> < 0.05), but they were not remarkably different between DDP group and DDP + rhGH group or between control group and rhGH group. Cells of gastric cancer xenograft in S phase distinctly diminished in DDP group and DDP + rhGH group compared with control group and rhGH group (<i>p</i> < 0.05), but they were not statistically significant between DDP group and DDP + rhGH group or between control group and rhGH group. IGF-I and IGFBP-3 of blood serum of nude mice obviously rose, but ratio of IGF-I and IGFBP-3 obviously lowered in rhGH group and DDP + rhGH group compared with control group and DDP group (<i>p</i> < 0.05). Expressions of IGF-I mRNA and IGF-IR mRNA were not obviously different in all groups. But expression of IGFBP-3 mRNA obviously increased in rhGH group, DDP group and DDP + rhGH group compared with control group; meanwhile, expression of IGFBP-3 mRNA also obviously increased in DDP + rhGH group compared with control group, DDP group and rhGH group. Conclusion: Our results indicated rhGH in short-time use did not improve proliferation of human gastric cancer cells and its mechanism was possible that rhGH in short-time use raised simultaneously IGF-I and IGFBP-3 of blood serum and increased IGFBP-3 mRNA, but degraded ratio of IGF-I and IGFBP-3 of blood serum in human gastric cancer cells.
%K Human Growth Hormone
%K Stomach Neoplasm
%K Insulin-Like Growth Factor
%K Insulin-Like Growth Factor Binding Protein-3
%K RT-Polymerase Chain Reaction
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=52582