%0 Journal Article
%T Isolation, Identification, and Characterization of Cadmium Resistant Pseudomonas sp. M3 from Industrial Wastewater
%A Syed Zaghum Abbas
%A Mohd Rafatullah
%A Norli Ismail
%A Japareng Lalung
%J Journal of Waste Management
%D 2014
%I Hindawi Publishing Corporation
%R 10.1155/2014/160398
%X The present study deals with the isolation, identification, and characterization of the cadmium resistant bacteria from wastewater collected from industrial area of Penang, Malaysia. The isolate was selected based on high level of the cadmium and antibiotic resistances. On the basis of morphological, biochemical characteristics, 16S rDNA gene sequencing and phylogeny analysis revealed that the strain RZCd1 was authentically identified as Pseudomonas sp. M3. The industrial isolate showed more than 70% of the cadmium removal in log phase. The cadmium removal capacity of strain RZCd1 was affected by temperature and pH. At pH 7.0 and 35¡ãC, strain RZCd1 showed maximum cadmium removal capacity. The minimal inhibitory concentration of strain RZCd1 against the cadmium was 550£¿¦Ìg/mL. The resistance against the cadmium was associated with resistance to multiple antibiotics: amoxicillin, penicillin, cephalexin, erythromycin, and streptomycin. The strain RZCd1 also gave thick bands of proteins in front of 25£¿kDa in cadmium stress condition after 3 h of incubation. So the identified cadmium resistant bacteria may be useful for the bioremediation of cadmium contaminated industrial wastewater. 1. Introduction Among the list of heavy metals, the cadmium needs special attention because it is identified as significant pollutant due to its high solubility and toxicity in the water [1, 2]. It is one of the most toxic pollutants of the surface soil layer, released into the environment by mining and smelting activities, atmospheric deposition from metallurgical industries, incineration of plastics and batteries, land application of sewage sludge, and burning of fossil fuels [3]. The wastewaters from the industries and sewage sludge applications have permanent toxic effects to human and the environment. Both terrestrial and aquatic environments have been greatly affected by the cadmium pollution [4]. In human, it affects cell proliferation, differentiation, apoptosis and increases oncogene activation to carcinogenesis. It also causes vertebral osteoporosis and fractures, toxicity to neuron, aging, and peripheral arterial disease. It selectively accumulates in pancreas, bones, renal liver, lungs, and kidneys [5, 6]. The water pollution caused by the cadmium has received increasing attention worldwide. Many conventional approaches have been considered for the removal of cadmium from industrial wastewater, mainly including chemical precipitation, ion exchange, membrane technology, and adsorption [7, 8]. In the field of bioremediation, simpler and relatively inexpensive ways are
%U http://www.hindawi.com/journals/jwm/2014/160398/