%0 Journal Article
%T Comparative Analysis of Sorghum bicolor Proteome in Response to Drought Stress and following Recovery
%A Christoph Jedmowski
%A Ahmed Ashoub
%A Tobias Beckhaus
%A Thomas Berberich
%A Michael Karas
%A Wolfgang Br¨¹ggemann
%J International Journal of Proteomics
%D 2014
%I Hindawi Publishing Corporation
%R 10.1155/2014/395905
%X The adaptive response of Sorghum bicolor landraces from Egypt to drought stress and following recovery was analyzed using two-dimensional difference gel electrophoresis, 2D-DIGE. Physiological measurements and proteome alterations of accession number 11434, drought tolerant, and accession number 11431, drought sensitive, were compared to their relative control values after drought stress and following recovery. Differentially expressed proteins were analysed by Matrix assisted laser desorption ionisation time-of-flight mass spectrometry, MALDI-TOF-MS. Alterations in protein contents related to the energy balance, metabolism (sensu Mewes et al. 1997), and chaperons were the most apparent features to elucidate the differences between the drought tolerant and sensitive accessions. Further alterations in the levels of proteins related to transcription and protein synthesis are discussed. 1. Introduction Abiotic stresses like drought, salinity, flooding, temperature extremes, and improper agricultural techniques have a negative impact on final yield of cultivated plants [1]. These stressors apart or combined can result in yield reduction of up to 80% [2]. Water availability is considered a major limitation for plant production [3]. Drought triggers the signal transduction of the phytohormone abscisic acid, ABA, a key hormone involved in stomata closure to reduce transpiration. Drought also suppresses cell growth and photosynthesis efficiency, increases respiration [4], and induces several other genes involved in the response to abiotic stresses [5]. Several drought-inducible genes involved in a broad range of functions have been identified by molecular and genomic analyses in Arabidopsis, rice, and other plants [5, 6]. However, the level of a specific mRNA does not always correlate well with the level of proteins. An mRNA produced in abundance may be degraded rapidly or translated inefficiently, resulting in a nonproportional abundance of mRNA and protein. Moreover, out of a given pool of mRNA, only a fraction is recruited further into the polyribosome assembly for translation [7]. Further, many transcripts give rise to more than one protein, through alternative splicing or alternative posttranscriptional modifications. Additionally, many proteins experience posttranslational modifications that profoundly affect their activities. Drought-inducible genes are classified into two groups. The first group includes proteins that function in abiotic stress tolerance such as chaperones, late embryogenesis abundant (LEA) proteins, osmotin, mRNA-binding proteins, key
%U http://www.hindawi.com/journals/ijpro/2014/395905/