%0 Journal Article
%T 液相色谱串联质谱法测定进出口水产品中亚甲基蓝药物残留<br>Determination of Methylene Blue Drug Residues in Import and Export Aquatic Products by Liquid Chromatography-Tandem Mass Spectrometry
%A 殷居易
%A 陈杰
%A 汤海青
%A 莫燕霞
%A 顾晓俊
%A 陈梅珍
%A 吴维尔
%A 何卫敏
%J Hans Journal of Food and Nutrition Science
%P 73-77
%@ 2166-6121
%D 2013
%I Hans Publishing
%R 10.12677/HJFNS.2013.24014
%X <div style=\"text-align:justify;\">
	<span style=\"line-height:1.5;\">运用高效液相色谱</span><span style=\"line-height:1.5;\">–</span><span style=\"line-height:1.5;\">电喷雾电离源串联四极杆质谱</span><span style=\"line-height:1.5;\">(HPLC-ESI(+)</span><span style=\"line-height:1.5;\">-</span><span style=\"line-height:1.5;\">MS/MS)</span><span style=\"line-height:1.5;\">法对进出口水产品中亚甲基蓝药物残留进行高灵敏分析。样品经过乙腈溶剂提取后，用中性氧化铝柱固相萃取柱化，洗脱收集后蒸干，残留用定容液溶解并过</span><span style=\"line-height:1.5;\">0.45 μm</span><span style=\"line-height:1.5;\">滤膜过滤，样品溶液供液相色谱</span><span style=\"line-height:1.5;\">–</span><span style=\"line-height:1.5;\">串联质谱仪测定。用</span><span style=\"line-height:1.5;\">HPLC-MS/MS</span><span style=\"line-height:1.5;\">在正离子模式下进行多反应监测</span><span style=\"line-height:1.5;\">(MRM)</span><span style=\"line-height:1.5;\">测定，根据校准曲线和外标法定量。电喷雾电离源正离子</span><span style=\"line-height:1.5;\">MRM</span><span style=\"line-height:1.5;\">模式检测：</span><span style=\"line-height:1.5;\">m/z 285.1/ 269.1</span><span style=\"line-height:1.5;\">；</span><span style=\"line-height:1.5;\">285.1/241.0</span><span style=\"line-height:1.5;\">。方法测定低限</span><span style=\"line-height:1.5;\">(LOQ</span><span style=\"line-height:1.5;\">, </span><span style=\"line-height:1.5;\">S/N </span><span style=\"line-height:1.5;\">&gt; </span><span style=\"line-height:1.5;\">10)</span><span style=\"line-height:1.5;\">为</span><span style=\"line-height:1.5;\">0.1 μg/kg</span><span style=\"line-height:1.5;\">；线性范围：</span><span style=\"line-height:1.5;\">0.5</span><span style=\"line-height:1.5;\">~</span><span style=\"line-height:1.5;\">50 μg/L</span><span style=\"line-height:1.5;\">内峰面积与浓度成良好线性</span><span style=\"line-height:1.5;\">(R2 </span><span style=\"line-height:1.5;\">&gt; </span><span style=\"line-height:1.5;\">0.999)</span><span style=\"line-height:1.5;\">。方法平均回收率范围在</span><span style=\"line-height:1.5;\">87.6%</span><span style=\"line-height:1.5;\">~</span><span style=\"line-height:1.5;\">111.2%</span><span style=\"line-height:1.5;\">，</span><span style=\"line-height:1.5;\">RSD</span><span style=\"line-height:1.5;\">范围在</span><span style=\"line-height:1.5;\">4.0%</span><span style=\"line-height:1.5;\">~</span><span style=\"line-height:1.5;\">10.2%</span><span style=\"line-height:1.5;\">。&lt;br/</span><span style=\"line-height:1.5;\">&gt;</span><span style=\"line-height:1.5;\">In this study, a HPLC-ESI(+)-MS/MS methodology is used for the high sensitive determination of drug resi- due of methylene blue (MB) in import and export aquatic products. The sample was extracted by the acetonitrile and operated with a neutral
%K 水产品；亚甲基蓝；液相色谱–串联质谱法；残留<br>Aquatic Products
%K Methylene Blue (MB)
%K LC-ESI(+)-MS/MS
%K Residues
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=12737