%0 Journal Article
%T Anti-Inflammatory and Analgesic Effects of the Marine-Derived Compound Comaparvin Isolated from the Crinoid Comanthus bennetti
%A Li-Chai Chen
%A Yen-You Lin
%A Yen-Hsuan Jean
%A Yi Lu
%A Wu-Fu Chen
%A San-Nan Yang
%A Hui-Min David Wang
%A Ing-Yang Jang
%A I-Ming Chen
%A Jui-Hsin Su
%A Ping-Jyun Sung
%A Jyh-Horng Sheu
%A Zhi-Hong Wen
%J Molecules
%P 14667-14686
%D 2014
%I MDPI AG
%R 10.3390/molecules190914667
%X To date, no study has been conducted to explore the bioactivity of the crinoid Comanthus bennetti. Here we report the anti-inflammatory properties of comaparvin (5,8-dihydroxy-10-methoxy-2-propylbenzo[h]chromen-4-one) based on in vivo experiments. Our preliminary screening for anti-inflammatory activity revealed that the crude extract of Comanthus bennetti significantly inhibited the expression of pro-inflammatory proteins in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophage cells. Comaparvin isolated from crinoids significantly decreased the expression of inducible nitric oxide synthase (iNOS) protein and mRNA in LPS-stimulated macrophage cells. Moreover, our results showed that post-treatment with comaparvin significantly inhibited mechanical allodynia, thermal hyperalgesia and weight-bearing deficits in rats with carrageenan-induced inflammation. Comaparvin also attenuated leukocyte infiltration and iNOS protein expression in carrageenan-induced inflamed paws. These results suggest that comaparvin is a potential anti-inflammatory therapeutic agent against inflammatory pain.
%K comaparvin
%K crinoids
%K Comanthus bennetti
%K inducible nitric oxide synthase
%K lipopolysaccharide
%K carrageenan
%U http://www.mdpi.com/1420-3049/19/9/14667