%0 Journal Article
%T Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract
%A Neelesh Sharma
%A Kalpa W. Samarakoon
%A Rajendra Gyawali
%A Yang-Ho Park
%A Sung-Jin Lee
%A Sung Jong Oh
%A Tae-Hoon Lee
%A Dong Kee Jeong
%J Molecules
%P 14567-14581
%D 2014
%I MDPI AG
%R 10.3390/molecules190914567
%X This study evaluated the chemical composition, antioxidant, anti-inflammatory and anticancer activities of a Euphorbia hirta L. extract. The antioxidant activities of whole E. hirta ethanol extract were determined by electron spin resonance spectrophotometric analysis of 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl, and alkyl radical levels and by using an online high-performance liquid chromatography (HPLC)-2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay. The E. hirta ethanol extract (0.5 mg/mL) exhibited DPPH-scavenging activity of 61.19% ¡À 0.22%, while the positive control (0.5 mg/mL ascorbic acid) had 100% ¡À 0.22% activity. The concentration of the extract required to trap 50% of DPPH (IC 50) was 0.205 mg/mL. Online HPLC analysis of the extract also showed strong antioxidant activity. The anti-inflammatory activity of the E. hirta extract was assessed in lipopolysaccharide-induced RAW 264.7 macrophages. The anti-inflammatory activity was highest in the presence of 200 ¦Ìg/mL E. hirta extract, and nitric oxide production was decreased significantly ( p < 0.05). The extract also showed selective anticancer activity at a concentration of 100 ¦Ìg/mL ( p < 0.05). These results indicated that E. hirta may warrant further investigation for the development of antioxidant, anti-inflammatory, and anticancer herbal medications.
%K Euphorbia hirta
%K chemical composition
%K online HPLC-ABTS+£¿ bioassay
%K antioxidant
%K anti-inflammatory
%K anticancer
%U http://www.mdpi.com/1420-3049/19/9/14567