%0 Journal Article
%T The Histochemical Characterization of the Glycoconjugates in the Epidermal Mucous Cells of the Red Californian Earthworm, Eisenia foetida
%A Kenan £¿inar
%A Mustafa £¿ztop
%A Emel Demirba£¿
%J Journal of Histology
%D 2014
%R 10.1155/2014/130497
%X The aim of this study was to characterize the nature and regional distribution of the glycoconjugates secreted by epidermal mucous cells in Eisenia foetida (Annelida). Specimens were divided into six regions from anterior to posterior. The histochemistry was carried out by using standard histochemical methods. Histochemical staining properties of glycoconjugates in epidermal mucous cells were determined regionally. The epidermis of all regions contained strong to stronger PAS (+) cells in various degrees. The epidermis of the first, fourth, fifth, and sixth regions had strong to stronger AB pH 2.5 (+) cells. On the contrary, all regions contained weak to moderate AB pH 0.5 and AB pH 1.0 (+) cells. Most of mucous cells in epidermis of the first region contained both PAS (+) and AB (+) mucosubstances. All regions included weaker to weak AF (+) cells. All regions featured KOH/PAS (+) cells, with a slight reduction in reaction intensity in the epidermis of the last three regions. In this context, the different staining patterns observed in epidermal mucous cells hinted at their functional roles with respect to production of mucus with different physical properties. This study provided comprehensive information about the regional distribution patterns of the glycoconjugates and an opportunity to compare their distributional patterns in other annelids. 1. Introduction Histological, histochemical, and ultrastructural studies have unveiled the existence of a variable and complex structure of the epidermis in annelids [1¨C3]. The epidermis of the annelids consists of a monolayered epithelium, which contains glandular, supporting, ciliated, and sensory cells. The epidermis is covered by a cuticle composed of collagen fibers embedded in a matrix [4]. The glandular cells in the surface epithelia secrete mucus that is rich in glycans, glycoproteins, and sialic acid residues [5¨C7]. In the annelids, this mucous secretion also plays a fundamental role in the formation of the ground substance of tubes, protection from dehydration, absorption of metabolites, and protection against parasites [4]. The glycoconjugates, which are essential components of the glycocalyx of many cell types, take part in many functions, including osmoregulation, cell to cell recognition, binding of hormones, protection of cells from phagocytosis and dehydration, differentiation, defense, and ion transport [8]. In some epithelial cells, which morphologically and functionally polarize in relation to different behaviours in ion transport [9], it is likely that a functional specialization of the
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