%0 Journal Article
%T DNA Barcoding on Bacteria: A Review
%A D. E. Lebonah
%A A. Dileep
%A K. Chandrasekhar
%A S. Sreevani
%A B. Sreedevi
%A J. Pramoda Kumari
%J Advances in Biology
%D 2014
%R 10.1155/2014/541787
%X Bacteria are omnipotent and they can be found everywhere. The study of bacterial pathogens has been happening from olden days to prevent epidemics, food spoilage, losses in agricultural production, and loss of lives. Modern techniques in DNA based species identification are considered. So, there is a need to acquire simple and quick identification technique. Hence, this review article covers the efficacy of DNA barcoding of bacteria. Routine DNA barcoding involves the production of PCR amplicons from particular regions to sequence them and these sequence data are used to identify or ¡°barcode¡± that organism to make a distinction from other species. 1. Introduction Nowadays DNA barcoding has become a justifiable tool for the assessment of global biodiversity patterns and it can allow diagnosis of known species to nontaxonomists [1]. DNA barcoding is a fast, accurate, and standardized method for species level identification, by using short DNA sequences. In 2003, Hebert from the University of Guelph, Ontario, Canada, proposed a new technique called DNA barcoding. Hebert and his associates published a paper entitled ¡°Biological identifications through DNA barcodes¡±. They proposed a new system of species identification, that is, the discovery of species by using a short segment of DNA from a standardized region of the genome. That DNA sequence can be used to identify different species. DNA barcoding is the standardized research that facilitates biodiversity studies like species identification and discovery. This technique helps researchers to understand genetic and evolutionary relationships by assembling molecular, morphological, and distributional data [2]. Species-level identification by DNA barcoding is usually adapted by the recovery of a short DNA sequence from a standard part of the genome [3]. The sequence of barcode from each unknown specimen was then compared with a library of reference barcode sequences obtained from individuals of recognized identity [4]. DNA barcoding is an obligatory tool for species detection and specimen identification [5]. Using standardized identification method is very advantageous for mapping of all the species on Earth, especially when DNA sequencing technology is inexpensively obtainable. The term ¡°DNA barcode¡± suggests that the standardized DNA sequences can identify taxa in the same way as the 11-digit Universal Product Code identifies retail products in market [6]. Lambert et al. (2005) scrutinized the opportunity of using DNA barcoding to measure the past diversity of the Earth¡¯s biota [7]. The barcode of life data
%U http://www.hindawi.com/journals/ab/2014/541787/